Dataset Information

Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality.

ABSTRACT: Silver nanoparticles (AgNPs) have become known as a broad-spectrum antimicrobial agent. The antimicrobial activity of AgNPs is dependent on the particle size and the dispersion status. In this study, a simple and effective approach is developed for sequestering the biosynthesized AgNPs in silica composites during the gel formation of MCM-41. Composites with different Ag concentrations of 0.034% (Ag1@MCM-41), 0.151% (Ag2@MCM-41), and 0.369% (Ag3@MCM-41) are synthesized and then heated at 400 °C to produce Ag1@MCM-41H, Ag2@MCM-41H, and Ag3@MCM-41H, respectively. The samples are characterized by flame atomic absorption spectrometry, Fourier-transform infrared spectroscopy, X-ray diffraction, N₂ physisorption, scanning electron microscopy, transmission electron microscopy, and thermogravimetric analysis. The AgNPs are confirmed to be highly dispersed in the amorphous silica framework. The antimicrobial activity of the AgNP-silica samples is investigated against Staphylococcus aureus, Escherichia coli, and Candida albicans using the cup-plate and the plate-count techniques. The results show an excellent antimicrobial effect of these samples against the studied microorganisms. Importantly, the AgNP-silica samples are found to be stable up to 58 months under ambient conditions. These stable and powerful antimicrobial composites provide a more practical and effective strategy for combating biomedical pathogens and public health threats.

SUBMITTER: Abou-El-Sherbini KS

PROVIDER: S-EPMC6607213 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality.

Abou-El-Sherbini Khaled S KS Amer Mohey H A MHA Abdel-Aziz Mohamed S MS Hamzawy Esmat M A EMA Sharmoukh Walid W Elnagar Mohamed M MM

Global challenges (Hoboken, NJ) 20180819 10

Silver nanoparticles (AgNPs) have become known as a broad-spectrum antimicrobial agent. The antimicrobial activity of AgNPs is dependent on the particle size and the dispersion status. In this study, a simple and effective approach is developed for sequestering the biosynthesized AgNPs in silica composites during the gel formation of MCM-41. Composites with different Ag concentrations of 0.034% (Ag1@MCM-41), 0.151% (Ag2@MCM-41), and 0.369% (Ag3@MCM-41) are synthesized and then heated at 400 °C t ...[more]

PMID: 31565310

Similar Datasets

Project description:The sustained release of antimicrobial therapeutics for wound dressing has become an attractive design strategy for prolonging the timespan of wound dressings and for reducing the risk of chronic wound infection. Recently, cellulose-based membrane has become a preferred option of wound dressings for the treatment of burn wounds and skin ulcers. In this work, novel cellulose membrane incorporated with mesoporous silica particles (SBA-15) was developed as an antimicrobial wound dressing with desirable sustained release functionality for targeting persistent bacterial pathogens. Attributed to a coated layer of calcium carbonate (CaCO3), SBA-15 particles were free from corrosion in alkaline condition during the preparation of cellulose-based composite membranes. SEM, TEM and BET results showed that the morphology, specific surface area, pore size and pore volume of pristine SBA-15 were preserved after the incorporation of CaCO3-coated SBA-15 into the cellulose matrix, while the mesoporous structure of SBA-15 was significantly disrupted without the use of CaCO3 coating. The resultant composite membranes containing 30 wt% SBA-15 (denoted as CM-Ca2-SBA(30%)) achieved 3.6 wt% of antimicrobial drug loading. Interestingly, CM-Ca2-SBA(30%) demonstrated the sustained release property of chloramphenicol for 270 h, driven by a two-stage drug release processes of SBA-15/cellulose. The water vapor permeability (WVTR) and swelling properties of composite membranes were shown to have complied with the primary requirements of wound dressing. Antibacterial assays revealed that strong antibacterial activities (144 h) of the composite membranes against Staphylococcus aureus and Eschericia coli were achieved. All results displayed that the strategy of coating silica with CaCO3 helps to obtain cellulose-silica composite membranes with desirable sustained release profiles and strong antibacterial activities. The antibacterial SBA-15/cellulose composite membranes show potential for the application of wound dressing.

Project description:A reliable suspension-based platform for scaling engineered cardiac tissue (ECT) production from human induced pluripotent stem cells (hiPSCs) is crucial for regenerative therapies. Here, we compared the production and functionality of ECTs formed using our scaffold-based, engineered tissue microsphere differentiation approach with those formed using the prevalent scaffold-free aggregate platform. We utilized a microfluidic system for the rapid (1 million cells/min), high density (30, 40, 60 million cells/ml) encapsulation of hiPSCs within PEG-fibrinogen hydrogel microspheres. HiPSC laden-microspheres and aggregates underwent suspension-based cardiac differentiation in chemically defined media. In comparison to aggregates, microspheres maintained consistent size and shape initially, over time, and within and between batches. Initial size and shape coefficients of variation for microspheres were eight and three times lower, respectively, compared to aggregates. On day 10, microsphere cardiomyocyte (CM) content was 27% higher and the number of CMs per initial hiPSC was 250% higher than for aggregates. Contraction and relaxation velocities of microspheres were four and nine times higher than those of aggregates, respectively. Microsphere contractile functionality also improved with culture time, whereas aggregate functionality remained unchanged. Additionally, microspheres displayed improved β-adrenergic signaling responsiveness and uniform calcium transient propagation. Transcriptomic analysis revealed that while both microspheres and aggregates demonstrated similar gene regulation patterns associated with cardiomyocyte differentiation, heart development, cardiac muscle contraction, and sarcomere organization, the microspheres exhibited more pronounced transcriptional changes over time. Taken together, these results highlight the capability of the microsphere platform for scaling up biomanufacturing of ECTs in a suspension-based culture platform.

Dataset Information

Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality.

Publications

Encapsulation of Biosynthesized Nanosilver in Silica Composites for Sustainable Antimicrobial Functionality.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets