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ABSTRACT: Background
Adipose-derived mesenchymal stem cells (ADMSCs) can promote healing and inhibit inflammation/immune response in local tissues, while the detailed mechanism remains unknown.Results
ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (24 hours cultured) was collected. The CM was added to the M? culture system with lipopolysaccharide (LPS) or IL-4/IL-13 or blank. And those M? cultures without adding CM were used as controls. A series of classification markers and signaling pathways for M? polarization were detected by using flow cytometry, RT-PCR, and western blotting. Furthermore, the cell viability of all the groups was detected by CCK8 assay. After CM induction in different groups, M1-M? markers and M2a-M? were decreased; however, M2b/c-M? markers increased. STAT3/SOCS3 and STAT6/IRF4 were suppressed in all 3 CM-treated groups. Moreover, the cell viability of all 3 groups which were induced by CM significantly increased as compared to that of the control groups without adding CM.Conclusion
ADMSCs can induce nonactivated macrophage and M1-M? into M2b/c-M?. Downregulation of the STAT3 and STAT6 pathway may involve in this process. This data shows that the anti-inflammatory role of ADMSC in local tissues may be partly due to their effect on M? to M2b/c-M?.
SUBMITTER: Sun M
PROVIDER: S-EPMC6607735 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Sun Meng M Sun Linxiao L Huang Chongchu C Chen Bi-Cheng BC Zhou Zhenxu Z
Journal of immunology research 20190619
<h4>Background</h4>Adipose-derived mesenchymal stem cells (ADMSCs) can promote healing and inhibit inflammation/immune response in local tissues, while the detailed mechanism remains unknown.<h4>Results</h4>ADMSCs and peritoneal macrophages were collected from C57BL/6 mice. The culture medium (CM) from ADMSCs (24 hours cultured) was collected. The CM was added to the M<i>φ</i> culture system with lipopolysaccharide (LPS) or IL-4/IL-13 or blank. And those M<i>φ</i> cultures without adding CM were ...[more]