Ontology highlight
ABSTRACT:
SUBMITTER: Rooijers K
PROVIDER: S-EPMC6609448 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Nature biotechnology 20190617 7
Protein-DNA interactions are critical to the regulation of gene expression, but it remains challenging to define how cell-to-cell heterogeneity in protein-DNA binding influences gene expression variability. Here we report a method for the simultaneous quantification of protein-DNA contacts by combining single-cell DNA adenine methyltransferase identification (DamID) with messenger RNA sequencing of the same cell (scDam&T-seq). We apply scDam&T-seq to reveal how genome-lamina contacts or chromati ...[more]