Ontology highlight
ABSTRACT:
SUBMITTER: Ghani K
PROVIDER: S-EPMC6610700 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Ghani Karim K Boivin-Welch Michael M Roy Sylvie S Dakiw-Piaceski Angela A Barbier Martin M Pope Elena E Germain Lucie L Caruso Manuel M
Molecular therapy. Methods & clinical development 20190607
The γ-retroviral vector is a gene delivery vehicle that is commonly used in gene therapy. Despite its efficacy, its strong enhancers contributed to malignant transformations in some hematopoietic stem cell (HSC) gene therapy trials. A safer version without viral enhancers (SIN) is available, but its production is cumbersome, as high titers can only be obtained in transient transfection. Our aim was to develop a system that could easily generate high-titer SIN vectors from stable producer cells. ...[more]