Dataset Information

Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica.

ABSTRACT:

Background

Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophytic fungal strains for enhanced biodiesel production are rare. In this study, the interaction between P. indica and Parachlorella kessleri-I, a marine algal strain, was assessed at metabolic level.

Results

In association with an endophytic fungus, the algal biomass enhanced from 471.6 to 704 mg/L, and the fatty acid methyl ester (FAME) profile of P. kessleri-I increased substantially. In case of FAME profile of co-cultured P. kessleri-I, two essential components of biodiesel, i.e. elaidic acid and oleic acid, increased by 1.4- and 1.8-fold, respectively. To ascertain changes in the metabolic profile of P. kessleri-I by P. indica co-culture, gas chromatography-mass spectrometry (GC-MS)-based untargeted metabolomics study was performed to identify the metabolites involved; and differential nature of the essential metabolites was also confirmed using HPLC and LC-MS. Significant modulation of the bioactive metabolites such as succinate, oxo-propanoate, l-alanine, glutamate, acetate and 1,2 propanediol, hydroxy butane was observed.

Conclusion

The metabolites like glutamate and succinate that usually belong to the GABA shunt pathway were observed to be upregulated. The pathway links nitrogen metabolism and carbon metabolism, thus influencing the growth and lipid profile of the algae. These differential metabolites thus indicated the important commensal association between the endophytic fungus and autotrophic marine alga, and established that endophytic fungus can be handy for the sustainability of algal biofuel industries.

SUBMITTER: Bhatnagar VS

PROVIDER: S-EPMC6613240 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Amelioration of biomass and lipid in marine alga by an endophytic fungus <i>Piriformospora indica</i>.

Bhatnagar Vipul Swarup VS Bandyopadhyay Prasun P Rajacharya Girish H GH Sarkar Sharanya S Poluri Krishna Mohan KM Kumar Shashi S

Biotechnology for biofuels 20190708

<h4>Background</h4>Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus <i>Piriformospora indica.</i> However, studies involving the co-culture of alga with endophytic fungal strains for enhanced biodiesel production are rare. In this study, the interaction between <i>P. indica</i> and <i>Parachlorella kessleri</i>-I, a marine algal strain, was assessed at metabolic level.<h4>Results</h4>In association with an endophytic fun ...[more]

PMID: 31316583

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Project description:Banana (Musa spp.), one of the most important fruits worldwide, is generally cold sensitive. In this study, by using the cold-sensitive banana variety Tianbaojiao (Musa acuminate) as the study material, we investigated the effects of Piriformospora indica on banana cold resistance. Seedlings with and without fungus colonization were subjected to 4 °C cold treatment. The changes in plant phenotypes, some physiological and biochemical parameters, chlorophyll fluorescence parameters, and the expression of eight cold-responsive genes in banana leaves before and after cold treatment were measured. Results demonstrated that P. indica colonization reduced the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased the activities of superoxide dismutase (SOD) and catalase (CAT) and the contents of soluble sugar (SS) and proline. Noteworthily, the CAT activity and SS content in the leaves of P. indica-colonized banana were significant (p < 0.05). After 24 h cold treatment, the decline in maximum photochemistry efficiency of photosystem II (Fv/Fm), photochemical quenching coefficient (qP), efficient quantum yield [Y(II)], and photosynthetic electron transport rate (ETR) in the leaves of P. indica-colonized banana was found to be lower than in the non-inoculated controls (p < 0.05). Moreover, although the difference was not significant, P. indica colonization increased the photochemical conversion efficiency and electron transport rate and alleviated the damage to the photosynthetic reaction center of banana leaves under cold treatment to some extent. Additionally, the expression of the most cold-responsive genes in banana leaves was significantly induced by P. indica during cold stress (p < 0.05). It was concluded that P. indica confers banana with enhanced cold resistance by stimulating antioxidant capacity, SS accumulation, and the expression of cold-responsive genes in leaves. The results obtained from this study are helpful for understanding the P. indica-induced cold resistance in banana.