Dataset Information

Invasive pulmonary infection due to Thermoascus crustaceus in a kidney transplant recipient.

ABSTRACT: The first case of invasive pulmonary infection due to the thermophilic fungus Thermoascus crustaceus in a kidney transplant recipient is described. For the identification of the fungal isolate, morphological aspects and molecular analysis have been used. The case report emphasizes this fungal species as an opportunistic human pathogen and underlines the importance of an accurate laboratory diagnosis for the correct management of the patient.

SUBMITTER: Mares M

PROVIDER: S-EPMC6613454 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Invasive pulmonary infection due to <i>Thermoascus crustaceus</i> in a kidney transplant recipient.

Mareș Mihai M Moroti-Constantinescu Valentina-Ruxandra VR Voroneanu Luminița L Doroftei Florica F Covic Adrian A Mederle Ovidiu-Alexandru OA

Infection and drug resistance 20190703

The first case of invasive pulmonary infection due to the thermophilic fungus <i>Thermoascus crustaceus</i> in a kidney transplant recipient is described. For the identification of the fungal isolate, morphological aspects and molecular analysis have been used. The case report emphasizes this fungal species as an opportunistic human pathogen and underlines the importance of an accurate laboratory diagnosis for the correct management of the patient. ...[more]

PMID: 31308712

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Project description:Noroviruses are the leading cause of acute gastroenteritis, and they can affect humans of all age groups. In immunocompromised patients, norovirus infections can develop into chronic diarrhea or show prolonged asymptomatic virus shedding. Chronic norovirus infections are frequently reported for solid organ transplant recipients, with rapid intrahost norovirus evolution seen. In this report, we describe a case of chronic norovirus infection in an immunocompromised patient who was followed up for over 5 years. The purpose of the study was to specify the norovirus evolution in a chronically infected immunocompromised host and identify possible selection sites in norovirus capsid protein. During the follow-up period, 25 sequential stool samples were collected and nine of them were selected to generate amplicons covering viral RNA-dependent RNA polymerase (RdRp) and viral capsid protein (VP1) genes. Amplicons were sequenced using next-generation sequencing. Single nucleotide polymorphisms were defined, which demonstrated a nearly 3-fold greater mutation rate in the VP1 genome region compared to the RdRp genome region (7.9 vs. 2.8 variable sites/100 nucleotides, respectively). This indicates that mutations in the virus genome were not accumulated randomly, but are rather the result of mutant selection during the infection cycle. Using ShoRAH software we were able to reconstruct haplotypes occurring in each of the nine selected samples. The deduced amino-acid haplotype sequences were aligned and the positions were analyzed for selective pressure using the Datamonkey program. Only 12 out of 25 positive selection sites were within the commonly described epitopes A, B, C, and D of the VP1 protein. New positive selection sites were determined that have not been described before and might reflect adaptation of the norovirus toward optimal histo-blood-group antigen binding, or modification of the norovirus antigenic properties. These data provide new insights into norovirus evolutionary dynamics and indicate new putative epitope "hot-spots" of modified and optimized norovirus-host interactions.

Dataset Information

Invasive pulmonary infection due to Thermoascus crustaceus in a kidney transplant recipient.

Publications

Invasive pulmonary infection due to <i>Thermoascus crustaceus</i> in a kidney transplant recipient.

Similar Datasets

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