Mapping Molecular Structure of Protein Locating on Nanoparticles with Limited Proteolysis.
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ABSTRACT:
SUBMITTER: Duan Y
PROVIDER: S-EPMC6613589 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Mapping Molecular Structure of Protein Locating on Nanoparticles with Limited Proteolysis.
Duan Yaokai Y Liu Yang Y Coreas Roxana R Zhong Wenwan W
Analytical chemistry 20190307 6
The molecular structure of a protein could be altered when it is attached to nanoparticles (NPs), affecting the performance of NPs present in biological systems. Limited proteolysis coupled with LC-MS/MS could reveal the changes in protein structure when it binds to a variety of entities, including macro-molecules and small drugs, but it has not yet been applied to study protein-NP interaction. Herein, adsorption of proteins, transferrin, and catalase on the polystyrene (PS) or iron oxide (IO) N ...[more]
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