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Publisher Correction: The role of Patronin in Drosophila mitosis.

ABSTRACT: During production of the original article [1], there was a technical error that resulted in author corrections not being rendered in the PDF version of the article.

SUBMITTER: Pavlova GA

PROVIDER: S-EPMC6615211 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publisher Correction: The role of Patronin in Drosophila mitosis.

Pavlova Gera A GA Razuvaeva Alyona V AV Popova Julia V JV Andreyeva Evgeniya N EN Yarinich Lyubov A LA Lebedev Mikhail O MO Pellacani Claudia C Bonaccorsi Silvia S Somma Maria Patrizia MP Gatti Maurizio M Pindyurin Alexey V AV

BMC molecular and cell biology 20190708 1

During production of the original article [1], there was a technical error that resulted in author corrections not being rendered in the PDF version of the article. ...[more]

PMID: 31286886

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The role of Patronin in Drosophila mitosis.

Project description:BackgroundThe calmodulin-regulated spectrin-associated proteins (CAMSAPs) belong to a conserved protein family, which includes members that bind the polymerizing mcrotubule (MT) minus ends and remain associated with the MT lattice formed by minus end polymerization. Only one of the three mammalian CAMSAPs, CAMSAP1, localizes to the mitotic spindle but its function is unclear. In Drosophila, there is only one CAMSAP, named Patronin. Previous work has shown that Patronin stabilizes the minus ends of non-mitotic MTs and is required for proper spindle elongation. However, the precise role of Patronin in mitotic spindle assembly is poorly understood.ResultsHere we have explored the role of Patronin in Drosophila mitosis using S2 tissue culture cells as a model system. We show that Patronin associates with different types of MT bundles within the Drosophila mitotic spindle, and that it is required for their stability. Imaging of living cells expressing Patronin-GFP showed that Patronin displays a dynamic behavior. In prometaphase cells, Patronin accumulates on short segments of MT bundles located near the chromosomes. These Patronin "seeds" extend towards the cell poles and stop growing just before reaching the poles. Our data also suggest that Patronin localization is largely independent of proteins acting at the MT minus ends such as Asp and Klp10A.ConclusionOur results suggest a working hypothesis about the mitotic role of Patronin. We propose that Patronin binds the minus ends within MT bundles, including those generated from the walls of preexisting MTs via the augmin-mediated pathway. This would help maintaining MT association within the mitotic bundles, thereby stabilizing the spindle structure. Our data also raise the intriguing possibility that the minus ends of bundled MTs can undergo a limited polymerization.

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