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Investigation of candidate genes of non-syndromic cleft lip with or without cleft palate, using both case-control and family-based association studies.


ABSTRACT: OBJECTIVE:Non-syndromic cleft of the lip and/or palate (NSCL/P) is one of the most common polygenic diseases. In this study, both case-control and family-based association study were used to confirm whether the Single Nucleotide Polymorphisms (SNPs) were associated with NSCL/P. METHODS:A total of 37 nuclear families and 189 controls were recruited, whose blood DNA was extracted and subjected to genotyping of SNPs of 27 candidate genes by polymerase chain reaction-improved multiple ligase detection reaction technology (PCR-iMLDR). Case-control statistical analysis was performed using the SPSS 19.0. Haplotype Relative Risk (HRR), transmission disequilibrium test (TDT), and Family-Based Association Test (FBAT) were used to test for over-transmission of the target alleles in case-parent trios. The gene-gene interactions on NSCL/P were analyzed by Unphased-3.1.4. RESULTS:In case-control statistical analysis, only C14orf49 chr14_95932477 had statistically significant on genotype model (P?=?.03) and allele model (P?=?.03). Seven SNPs had statistically significant on TDT. None of 26 alleles has association with NSCL/P on FBAT. Some SNPs had haplotype-haplotype interactions and genotype-genotype interactions. CONCLUSION:C14orf49 chr14_95932477 was significantly different between cases and controls on genotype model and allele model by case-control design. Seven SNPs were significantly different on HRR. Four SNPs were significantly different on TDT.

SUBMITTER: Ge X 

PROVIDER: S-EPMC6617431 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Investigation of candidate genes of non-syndromic cleft lip with or without cleft palate, using both case-control and family-based association studies.

Ge Xing X   Hong Jia-Wei JW   Shen Jun-Yu JY   Li Zheng Z   Zhang Rui R   Wang Qi Q   Ding Zhen Z   Chen Gang G   Xu Li-Chun LC  

Medicine 20190601 26


<h4>Objective</h4>Non-syndromic cleft of the lip and/or palate (NSCL/P) is one of the most common polygenic diseases. In this study, both case-control and family-based association study were used to confirm whether the Single Nucleotide Polymorphisms (SNPs) were associated with NSCL/P.<h4>Methods</h4>A total of 37 nuclear families and 189 controls were recruited, whose blood DNA was extracted and subjected to genotyping of SNPs of 27 candidate genes by polymerase chain reaction-improved multiple  ...[more]

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