Noninvasive Mechanical Joint Loading as an Alternative Model for Osteoarthritic Pain.

ABSTRACT:

Objective

Mechanisms responsible for osteoarthritic (OA) pain remain poorly understood, and current analgesic therapies are often insufficient. This study was undertaken to characterize and pharmacologically test the pain phenotype of a noninvasive mechanical joint loading model of OA, thus providing an alternative murine model for OA pain.

Methods

The right knees of 12-week-old male C57BL/6 mice were loaded at 9N or 11N (40 cycles, 3 times per week for 2 weeks). Behavioral measurements of limb disuse and mechanical and thermal hypersensitivity were acquired before mechanical joint loading and monitored for 6 weeks postloading. The severity of articular cartilage lesions was determined postmortem with the Osteoarthritis Research Society International scoring system. To asses

SUBMITTER: Ter Heegde F 

PROVIDER: S-EPMC6618037 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature