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Light-Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein.


ABSTRACT: Light-induced pulsed EPR dipolar spectroscopic methods allow the determination of nanometer distances between paramagnetic sites. Here we employ orthogonal spin labels, a chromophore triplet state and a stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. We demonstrate that Zn-substitution of neuroglobin, to populate the Zn(II) protoporphyrin IX triplet state, makes it possible to perform light-induced pulsed dipolar experiments on hemeproteins, extending the use of light-induced dipolar spectroscopy to this large class of metalloproteins. The versatility of the method is ensured by the employment of different techniques: relaxation-induced dipolar modulation enhancement (RIDME) is applied for the first time to the photoexcited triplet state. In addition, an alternative pulse scheme for laser-induced magnetic dipole (LaserIMD) spectroscopy, based on the refocused-echo detection sequence, is proposed for accurate zero-time determination and reliable distance analysis.

SUBMITTER: Dal Farra MG 

PROVIDER: S-EPMC6618045 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Light-Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein.

Dal Farra Maria Giulia MG   Richert Sabine S   Martin Caterina C   Larminie Charles C   Gobbo Marina M   Bergantino Elisabetta E   Timmel Christiane R CR   Bowen Alice M AM   Di Valentin Marilena M  

Chemphyschem : a European journal of chemical physics and physical chemistry 20190321 7


Light-induced pulsed EPR dipolar spectroscopic methods allow the determination of nanometer distances between paramagnetic sites. Here we employ orthogonal spin labels, a chromophore triplet state and a stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. We demonstrate that Zn-substitution of neuroglobin, to populate the Zn(II) protoporphyrin IX triplet state, makes it possible to perform light-induced pulsed dipolar experiments on hemeproteins, exte  ...[more]

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