Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls.

ABSTRACT: Gravitropism is an adaptive response that orients plant growth parallel to the gravity vector. Asymmetric distribution of the phytohormone auxin is a necessary prerequisite to the tropic bending both in roots and shoots. During hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing cells to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization to the bottom cell membranes leads to the auxin accumulation at the lower side of the organ, initiating bending and, later, auxin feedback-mediated repolarization restores symmetric auxin distribution to terminate bending. Here, we performed a forward genetic screen to identify regulators of both PIN3 polarization events during gravitropic response. We searched for mutants with defective PIN

SUBMITTER: Rakusova H 

PROVIDER: S-EPMC6618169 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature