A dual-model SERS and RRS analytical platform for Pb(II) based on Ag-doped carbon dot catalytic amplification and aptamer regulation.

ABSTRACT: Several carbon dots doping with diferent elements (Ca, Ag, Au) were fabricated and their catalytic properties had been investigated in this paper. It was found that the Ag-doped carbon dots (CD_Ag) had played a role of mimic enzyme on the reaction of HAuCl₄-H₂O₂ and generated nanogold particles with surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) effects. The aptamer (Apt) can be adsorbed on the CD_Ag surface and cause the catalysis weakening. When the target Pb(II) was added, it would combine with the Apt to produce firm complexes Pb-Apt and desorb CD_Ag, which caused its catalytic effect restore. The formed nanogold had a strong RRS peak (at 375?nm) and a high SERS peak (at 1615 cm^-1) in the presence of molecular probe (Victoria blue B, VBB). The dual-model signals of SERS and RRS increased linearly with Pb(II) concentration increase within the scope of 0.006-0.46 ?mol/L and 0.01-0.46 ?mol/L. And their detection limits respectively were 0.0032 ?mol/L and 0.0048 ?mol/L Pb(II).

SUBMITTER: Wang H 

PROVIDER: S-EPMC6620292 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature