Ontology highlight
ABSTRACT:
SUBMITTER: Zhang P
PROVIDER: S-EPMC6624853 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Nature methods 20181030 11
A fluorescent emitter simultaneously transmits its identity, location, and cellular context through its emission pattern. We developed smNet, a deep neural network for multiplexed single-molecule analysis to retrieve such information with high accuracy. We demonstrate that smNet can extract three-dimensional molecule location, orientation, and wavefront distortion with precision approaching the theoretical limit, and therefore will allow multiplexed measurements through the emission pattern of a ...[more]