Ontology highlight
ABSTRACT:
SUBMITTER: Wolock SL
PROVIDER: S-EPMC6625319 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Cell systems 20190403 4
Single-cell RNA-sequencing has become a widely used, powerful approach for studying cell populations. However, these methods often generate multiplet artifacts, where two or more cells receive the same barcode, resulting in a hybrid transcriptome. In most experiments, multiplets account for several percent of transcriptomes and can confound downstream data analysis. Here, we present Single-Cell Remover of Doublets (Scrublet), a framework for predicting the impact of multiplets in a given analysi ...[more]