Ontology highlight
ABSTRACT:
SUBMITTER: Azarkh M
PROVIDER: S-EPMC6625747 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Azarkh Mykhailo M Bieber Anna A Qi Mian M Fischer Jörg W A JWA Yulikov Maxim M Godt Adelheid A Drescher Malte M
The journal of physical chemistry letters 20190318 7
In-cell distance determination by electron paramagnetic resonance (EPR) spectroscopy reveals essential structural information about biomacromolecules under native conditions. We demonstrate that the pulsed EPR technique RIDME (relaxation induced dipolar modulation enhancement) can be utilized for such distance determination. The performance of in-cell RIDME has been assessed at Q-band using stiff molecular rulers labeled with Gd(III)-PyMTA and microinjected into Xenopus laevis oocytes. The overt ...[more]