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Long non-coding RNA LUCAT1/miR-5582-3p/TCF7L2 axis regulates breast cancer stemness via Wnt/?-catenin pathway.

ABSTRACT: BACKGROUND:The mechanism underlying breast cancer stem cell (BCSCs) characteristics remains to be fully elucidated. Accumulating evidence implies that long noncoding RNAs (lncRNAs) play a pivotal role in regulating BCSCs stemness. METHODS:LncRNA LUCAT1 expression was assessed in breast cancer tissues (n?=?151 cases) by in situ hybridization. Sphere-formation assay and colony formation assay were used to detect cell self-renewal and proliferation, respectively. RNA immunoprecipitation, RNA pull down and luciferase reporter assays were used to identify LUCAT1 and TCF7L2 as the direct target of miR-5582-3p. The activity of the Wnt/?-catenin pathway was analyzed by TOP/FOP-Flash reporter assays, western blot and immunohistochemistry (IHC). RESULTS:This study found LUCAT1 expression was related to tumor size (p?=?0.015), lymph node metastasis (p?=?0.002) and TNM staging (p?

SUBMITTER: Zheng A

PROVIDER: S-EPMC6626338 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Long non-coding RNA LUCAT1/miR-5582-3p/TCF7L2 axis regulates breast cancer stemness via Wnt/β-catenin pathway.

Zheng Ang A Song Xinyue X Zhang Lin L Zhao Lin L Mao Xiaoyun X Wei Minjie M Jin Feng F

Journal of experimental & clinical cancer research : CR 20190712 1

<h4>Background</h4>The mechanism underlying breast cancer stem cell (BCSCs) characteristics remains to be fully elucidated. Accumulating evidence implies that long noncoding RNAs (lncRNAs) play a pivotal role in regulating BCSCs stemness.<h4>Methods</h4>LncRNA LUCAT1 expression was assessed in breast cancer tissues (n = 151 cases) by in situ hybridization. Sphere-formation assay and colony formation assay were used to detect cell self-renewal and proliferation, respectively. RNA immunoprecipitat ...[more]

PMID: 31300015

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