Dataset Information

Comparison of the Substrate Preferences of ?3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.

ABSTRACT: Omega-3 long chain polyunsaturated fatty acids (?3 LC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5?3) and docosahexaenoic acid (DHA; 22:6?3) are important fatty acids for human health. These ?3 LC-PUFAs are produced from their ?3 precursors by a set of desaturases and elongases involved in the biosynthesis pathway and are also converted from ?6 LC-PUFA by omega-3 desaturases (?3Ds). Here, we have investigated eight ?3-desaturases obtained from a cyanobacterium, plants, fungi and a lower animal species for their activities and compared their specificities for various C18, C20 and C22 ?6 PUFA substrates by transiently expressing them in Nicotiana benthamiana leaves. Our results showed hitherto unreported activity of many of the ?3Ds on ?6 LC-PUFA substrates leading to their conversion to ?3 LC-PUFAs. This discovery could be important in the engineering of EPA and DHA in heterologous hosts.

SUBMITTER: Shrestha P

PROVIDER: S-EPMC6627408 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.

Shrestha Pushkar P Zhou Xue-Rong XR Vibhakaran Pillai Sapna S Petrie James J de Feyter Robert R Singh Surinder S

International journal of molecular sciences 20190622 12

Omega-3 long chain polyunsaturated fatty acids (ω3 LC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3) are important fatty acids for human health. These ω3 LC-PUFAs are produced from their ω3 precursors by a set of desaturases and elongases involved in the biosynthesis pathway and are also converted from ω6 LC-PUFA by omega-3 desaturases (ω3Ds). Here, we have investigated eight ω3-desaturases obtained from a cyanobacterium, plants, fungi and a lower anima ...[more]

PMID: 31234541

Similar Datasets

Project description:In the last decades, the development of new technologies applied to lipidomics has revitalized the analysis of lipid profile alterations and the understanding of the underlying molecular mechanisms of lipid metabolism, together with their involvement in the occurrence of human disease. Of particular interest is the study of omega-3 and omega-6 long chain polyunsaturated fatty acids (LC-PUFAs), notably EPA (eicosapentaenoic acid, 20:5n-3), DHA (docosahexaenoic acid, 22:6n-3), and ARA (arachidonic acid, 20:4n-6), and their transformation into bioactive lipid mediators. In this sense, new families of PUFA-derived lipid mediators, including resolvins derived from EPA and DHA, and protectins and maresins derived from DHA, are being increasingly investigated because of their active role in the "return to homeostasis" process and resolution of inflammation. Recent findings reviewed in the present study highlight that the omega-6 fatty acid ARA appears increased, and omega-3 EPA and DHA decreased in most cancer tissues compared to normal ones, and that increments in omega-3 LC-PUFAs consumption and an omega-6/omega-3 ratio of 2-4:1, are associated with a reduced risk of breast, prostate, colon and renal cancers. Along with their lipid-lowering properties, omega-3 LC-PUFAs also exert cardioprotective functions, such as reducing platelet aggregation and inflammation, and controlling the presence of DHA in our body, especially in our liver and brain, which is crucial for optimal brain functionality. Considering that DHA is the principal omega-3 FA in cortical gray matter, the importance of DHA intake and its derived lipid mediators have been recently reported in patients with major depressive and bipolar disorders, Alzheimer disease, Parkinson's disease, and amyotrophic lateral sclerosis. The present study reviews the relationships between major diseases occurring today in the Western world and LC-PUFAs. More specifically this review focuses on the dietary omega-3 LC-PUFAs and the omega-6/omega-3 balance, in a wide range of inflammation disorders, including autoimmune diseases. This review suggests that the current recommendations of consumption and/or supplementation of omega-3 FAs are specific to particular groups of age and physiological status, and still need more fine tuning for overall human health and well being.

Dataset Information

Comparison of the Substrate Preferences of ?3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.

Publications

Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets