Dataset Information

The Diversity, Multiplicity of Infection and Population Structure of P. falciparum Parasites Circulating in Asymptomatic Carriers Living in High and Low Malaria Transmission Settings of Ghana.

ABSTRACT: : Background: Diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. This study utilized 12 polymorphic microsatellite (MS) markers and the Msp2 marker to examine diversity, multiplicity of infection (MOI) as well as the population structure of parasites circulating in two sites separated by about 92 km and with varying malaria transmission intensities within the Greater Accra Region of Ghana.

Methods

The diversity and MOI of P. falciparum parasites in 160 non-symptomatic volunteers living in Obom (high malaria transmission intensity) and Asutsuare (low malaria transmission intensity) aged between 8 and 60 years was determined using Msp2 genotyping and microsatellite analysis.

Results

The prevalence of asymptomatic P. falciparum carriers as well as the parasite density of infections was significantly higher in Obom than in Asutsuare. Samples from Asutsuare and Obom were 100% and 65% clonal, respectively, by Msp2 genotyping but decreased to 50% and 5%, respectively, when determined by MS analysis. The genetic composition of parasites from Obom and Asutsuare were highly distinct, with parasites from Obom being more diverse than those from Asutsuare.

Conclusion

Plasmodium falciparum parasites circulating in Obom are genetically more diverse and distinct from those circulating in Asutsuare. The MOI in samples from both Obom and Asutsuare increased when assessed by MS analysis relative to MSP2 genotyping. The TA40 and TA87 loci are useful markers for estimating MOI in high and low parasite prevalence settings.

SUBMITTER: Abukari Z

PROVIDER: S-EPMC6628376 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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The Diversity, Multiplicity of Infection and Population Structure of <i>P. falciparum</i> Parasites Circulating in Asymptomatic Carriers Living in High and Low Malaria Transmission Settings of Ghana.

Abukari Zakaria Z Okonu Ruth R Nyarko Samuel B SB Lo Aminata C AC Dieng Cheikh C CC Salifu Samson P SP Gyan Ben A BA Lo Eugenia E Amoah Linda E LE

Genes 20190607 6

<b>:</b> Background: Diversity in <i>Plasmodium falciparum</i> poses a major threat to malaria control and elimination interventions. This study utilized 12 polymorphic microsatellite (MS) markers and the Msp2 marker to examine diversity, multiplicity of infection (MOI) as well as the population structure of parasites circulating in two sites separated by about 92 km and with varying malaria transmission intensities within the Greater Accra Region of Ghana.<h4>Methods</h4>The diversity and MOI o ...[more]

PMID: 31181699

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