Dataset Information

Brown seaweed (AquaArom) supplementation increases food intake and improves growth, antioxidant status and resistance to temperature stress in Atlantic salmon, Salmo salar.

ABSTRACT: Seaweeds represent a vast resource that remains underutilized as an ingredient in aquafeeds. Here we probed the effect of addition of AquaArom, a seaweed meal derived from brown seaweeds (Laminaria sp., kelp), to fish feed on growth performance, antioxidant capacity and temperature responsiveness of mitochondrial respiration. A commercial salmonid feed was mixed with 0 (control), 3, 6 and 10% seaweed and fed to Atlantic salmon (Salmo salar) smolts for 30 days. The smolts consumed more of the seaweed-supplemented food relative to the control and there were no mortalities. Compared with the control, the final fish weight, standard length, weight gain and SGR were higher in fish fed diets supplemented with the 3 and 10% seaweed, while growth performance for fish maintained on 6% seaweed remained neutral. Importantly, seaweed supplementation increased protein efficiency ratio (PER) and tended to improve food conversion ratio (FCR). Although the hepatosomatic and visceral indices did not change, whole gut and intestinal weights and lengths were higher in fish maintained on seaweed-supplemented diets suggesting increased retention time and a larger surface area for food digestion and nutrient absorption. Measurement of antioxidant status revealed that seaweed supplementation dose-dependently increased plasma total antioxidant capacity as well as the level of glutathione, and activities of catalase and superoxide dismutase in liver mitochondria. Moreover, seaweed supplementation reduced the effect of acute temperature rise on mitochondrial respiration and proton leak. Overall, these data suggest that AquaArom can be mixed with fish food up to 10% to increase food consumption and enhance growth performance, as well as to improve antioxidant capacity and alleviate adverse effects of stressors such as temperature in fish.

SUBMITTER: Kamunde C

PROVIDER: S-EPMC6629153 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Brown seaweed (AquaArom) supplementation increases food intake and improves growth, antioxidant status and resistance to temperature stress in Atlantic salmon, Salmo salar.

Kamunde Collins C Sappal Ravinder R Melegy Tarek Mostafa TM

PloS one 20190715 7

Seaweeds represent a vast resource that remains underutilized as an ingredient in aquafeeds. Here we probed the effect of addition of AquaArom, a seaweed meal derived from brown seaweeds (Laminaria sp., kelp), to fish feed on growth performance, antioxidant capacity and temperature responsiveness of mitochondrial respiration. A commercial salmonid feed was mixed with 0 (control), 3, 6 and 10% seaweed and fed to Atlantic salmon (Salmo salar) smolts for 30 days. The smolts consumed more of the sea ...[more]

PMID: 31306449

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Project description:The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp recordings of transport and presteady-state currents. Both PepT2a and PepT2b in the presence of Gly-Gln elicit pH-dependent and Na+ independent inward currents. The biophysical and kinetic analysis of the recorded currents defined the transport properties, confirming that the two Atlantic salmon PepT2 proteins behave as high-affinity/low-capacity transporters. The recent structures and the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2-type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es). KEY POINTS: Two slc15a2-type genes, slc15a2a and slc15a2b coding for PepT2-type peptide transporters were found in the Atlantic salmon. slc15a2a transcripts, widely distributed in the fish tissues, are abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Amino acids involved in vertebrate Slc15 transport function are conserved in PepT2a and PepT2b proteins. Detailed kinetic analysis indicates that both PepT2a and PepT2b operate as high-affinity transporters. The kinetic schemes and structures proposed for the mammalian models of PepT2 are suitable to explain the function of the two Atlantic salmon transporters.

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Dataset Information

Brown seaweed (AquaArom) supplementation increases food intake and improves growth, antioxidant status and resistance to temperature stress in Atlantic salmon, Salmo salar.

Publications

Brown seaweed (AquaArom) supplementation increases food intake and improves growth, antioxidant status and resistance to temperature stress in Atlantic salmon, Salmo salar.

Similar Datasets

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