Dataset Information

Detection of Microbial 16S rRNA Gene in the Serum of Patients With Gastric Cancer.

ABSTRACT: Aberrance in the blood bacterial microbiome has been identified and validated in several non-infectious diseases, including cancer. The occurrence and progression of gastric cancer has been found to be associated with alterations in the microbiome composition. However, the composition of the blood microbiome in patients with gastric cancer is not well-characterized. To test this hypothesis, we conducted a case-control study to investigate the microbiota compositions in the serum of patients with gastric cancer. The serum microbiome was investigated in patients with gastric cancer, atypical hyperplasia, chronic gastritis, and in healthy controls using 16S rRNA gene sequencing targeting the V1-V2 region. Our results revealed that the structure of the serum microbiome in gastric cancer was significantly different from all other groups, and alpha diversity decreased from the healthy control to patients with gastric cancer. The serum microbiome correlated significantly with tumor-node-metastasis (TNM) stage, lymphatic metastasis, tumor diameter, and invasion depth in gastric cancer. Three genera or species, namely, Acinetobacter, Bacteroides, Haemophilus parainfluenzae, were enriched in patients with gastric cancer, whereas Sphingomonas, Comamonas, and Pseudomonas stutzeri were enriched in the healthy control. Furthermore, the structure of serum microbiota differed between gastric cancer lymphatic metastasis and non-lymphatic metastasis. As a pilot investigation to characterizing the serum microbiome in gastric cancer, our study provided a foundation for improving our understanding of the role of microbiota in the pathogenesis of gastric cancer.

SUBMITTER: Dong Z

PROVIDER: S-EPMC6629868 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Detection of Microbial 16S rRNA Gene in the Serum of Patients With Gastric Cancer.

Dong Zhaogang Z Chen Bin B Pan Hongwei H Wang Ding D Liu Min M Yang Yongmei Y Zou Mingjin M Yang Junjie J Xiao Ke K Zhao Rui R Zheng Xin X Zhang Lei L Zhang Yi Y

Frontiers in oncology 20190709

Aberrance in the blood bacterial microbiome has been identified and validated in several non-infectious diseases, including cancer. The occurrence and progression of gastric cancer has been found to be associated with alterations in the microbiome composition. However, the composition of the blood microbiome in patients with gastric cancer is not well-characterized. To test this hypothesis, we conducted a case-control study to investigate the microbiota compositions in the serum of patients with ...[more]

PMID: 31338330

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Project description:Gastric cancer is the second most prevalent and deadly cancer in China. Microbiota play an important role in gastric tumorigenesis. However, the available microbial marker studies for gastric cancer do not have consistent results. We searched PubMed for 16S rRNA sequencing in relevant literature on Chinese patients from 1 January 2005 to 18 July 2022, and 16 original articles were finally obtained. Alpha diversity, beta diversity, and bacterial taxa were used to explore the differences in gastric microbiota. Linear discriminant analysis of effect size and a random forest model were used to find the combination of genera with the best diagnostic efficacy. Streptococcus, Pseudomonas, Fusobacterium, Selenomonas, Peptostreptococcus, and Prevotella showed significant differences between gastric cancer and non-gastric cancer, but a single genus performed poorly in identifying patients with gastric cancer. However, a combination of genera Streptococcus, Peptostreptococcus, Selenomonas, Pseudomonas, and Prevotella had excellent performance in screening gastric cancer with the median area under the curve values of 0.7525 (range: 0.5859-0.9350), 0.8818 (range: 0.7397-0.9533), and 0.7435 (range: 0.7131-0.8483) in the Matched, Unmatched, and Other groups, respectively. Therefore, the results indicated that this combination of genera has good diagnostic efficacy and wide applicability for patients with gastric cancer, which may provide new clues for the non-invasive diagnosis of gastric cancer. IMPORTANCE Gastric cancer is a significant and growing health problem in China. Studies have revealed significant differences in gastric microbiota between patients with gastric cancer and non-cancerous patients, suggesting that microbiota may play a role in tumorigenesis. In this meta-analysis, existing 16S rRNA microbial data were analyzed to find combinations consisting of five genera, which had good efficacy in distinguishing gastric cancer from non-cancerous patients in multiple types of samples. These results lend support to the use of microbial markers in detecting gastric cancer. Moreover, these biomarkers are plausible candidates for further mechanistic research into the role of the microbiota in tumorigenesis.

Project description:BackgroundDespite recent advances in studies on the gastric microbiome, the role of the non-Helicobacter pylori gastric microbiome in gastric carcinogenesis remains unclear. We evaluated the characteristics of the gastric microbiome and metagenomic functions in patients with IM.MethodsParticipants were classified into six groups according to disease status (chronic superficial gastritis [CSG], intestinal metaplasia [IM], and cancer) and H. pylori- infection status (H. pylori-positive and H. pylori-negative). The gastric microbiome was analyzed in mucosal tissues at the gastric antrum by 16S rRNA gene sequencing. Moreover, we assessed the metagenome including the type IV secretion system (T4SS) gene, as T4SS proteins are essential for transferring CagA from H. pylori- into the human gastric epithelium.ResultsAmong the 138 included patients, 48, 9, 23, 14, 12, and 32 were classified into the H. pylori-negative CSG, H. pylori-negative IM, H. pylori-negative cancer, H. pylori-positive CSG, H. pylori-positive IM, and H. pylori-positive cancer groups, respectively. Cyanobacteria were predominant in the H. pylori-negative CSG group compared to in the H. pylori-negative IM and H. pylori-negative cancer groups (H. pylori-negative CSG vs H. pylori-negative IM vs H. pylori-negative cancer: 14.0% vs 4.2% vs 0.04%, P < 0.001). In contrast, Rhizobiales were commonly observed in the H. pylori-negative IM group (H. pylori-negative CSG vs H. pylori-negative IM vs H. pylori-negative cancer: 1.9% vs 15.4% vs 2.8%, P < 0.001). The relative abundance of Rhizobiales increased as H. pylori-infected stomachs progressed from gastritis to IM. In the H. pylori-negative IM group, genes encoding T4SS were prevalent among the metagenome. Additionally, after H. pylori- eradication therapy, the gastric microbiome was similar to the microbiome observed after spontaneous clearance of H. pylori-.ConclusionsThe relative abundance of Rhizobiales was higher in patients with H. pylori-negative IM than in those with H. pylori-negative CSG or cancer. Additionally, T4SS genes were highly observed in the metagenome of patients with IM. Highly abundant T4SS proteins in these patients may promote gastric carcinogenesis.

Dataset Information

Detection of Microbial 16S rRNA Gene in the Serum of Patients With Gastric Cancer.

Publications

Detection of Microbial 16S rRNA Gene in the Serum of Patients With Gastric Cancer.

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