Dataset Information

Distribution and Succession Feature of Antibiotic Resistance Genes Along a Soil Development Chronosequence in Urumqi No.1 Glacier of China.

ABSTRACT: Primary succession of plant and microbial communities in the glacier retreating foreland has been extensively studied, but shifts of antibiotic resistance genes (ARGs) with the glacier retreating due to global warming remain elusive. Unraveling the diversity and succession features of ARGs in pristine soil during glacier retreating could contribute to a mechanistic understanding of the evolution and development of soil resistome. In this study, we quantified the abundance and diversity of ARGs along a 50-year soil development chronosequence by using a high-throughput quantitative PCR (HT-qPCR) technique. A total of 24 ARGs and two mobile genetic elements (MGEs) were detected from all the glacier samples, and the numbers of detected ARGs showed a unimodal pattern with an increasing trend at the early stage (0?8 years) but no significant change at later stages (17?50 years). The oprJ and mexF genes encoding multidrug resistance were the only two ARGs that were detected across all the succession ages, and the mexF gene showed an increasing trend along the succession time. Structural equation models indicated the predominant role of the intI1 gene encoding the Class 1 integron-integrase in shaping the variation of ARG profiles. These findings suggested the presence of ARGs in pristine soils devoid of anthropogenic impacts, and horizontal gene transfer mediated by MGEs may contribute to the succession patterns of ARGs during the initial soil formation stage along the chronosequence.

SUBMITTER: Shen JP

PROVIDER: S-EPMC6629927 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Distribution and Succession Feature of Antibiotic Resistance Genes Along a Soil Development Chronosequence in Urumqi No.1 Glacier of China.

Shen Ju-Pei JP Li Zong-Ming ZM Hu Hang-Wei HW Zeng Jun J Zhang Li-Mei LM Du Shuai S He Ji-Zheng JZ

Frontiers in microbiology 20190709

Primary succession of plant and microbial communities in the glacier retreating foreland has been extensively studied, but shifts of antibiotic resistance genes (ARGs) with the glacier retreating due to global warming remain elusive. Unraveling the diversity and succession features of ARGs in pristine soil during glacier retreating could contribute to a mechanistic understanding of the evolution and development of soil resistome. In this study, we quantified the abundance and diversity of ARGs a ...[more]

PMID: 31354668

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Project description:Microbes that produce phosphatases play an important role in the cycling of phosphorus (P), a key nutrient in soil development. We studied the development, compositional turnover, and environmental drivers of microbial communities carrying the phosphatase-encoding phoD gene (here called phoD communities) in the course of primary succession in the Hailuogou glacier chronosequence. We selected the pioneer species Populus purdomii Rehder as a model plant to study the communities in rhizosphere and bulk soils along the chronosequence. The bulk and rhizosphere soils hosted distinct phoD communities. Changes in the taxa Pseudomonas and Pleomorphomonas in the rhizosphere and Bradyrhizobium, Cupriavidus, and Pleomorphomonas in the bulk soil were associated with soil development. The plant development and soil property changes along the chronosequence were accompanied with changes in the phoD communities. Soil pH, soil organic carbon, and total nitrogen contents that are directly related to the plant development and litter input differences along the chronosequence were the main factors related to changes in community compositions. The community similarity decreased along the chronosequence, and the distance decay rate was higher in the bulk soil than in the rhizosphere. In summary, both in the rhizosphere and in bulk soils the phoD community succession was shaped by plant and soil development-related factors along the primary succession in the Hailuogou glacier chronosequence.IMPORTANCE Phosphorus was the key limiting nutrient for soil development during primary succession that occurred in alpine and high-latitude ecosystems with cold and humid climates. The interactions of functional microbiota involved in phosphorus cycling in the rhizosphere under different soil developmental stages along primary succession are still rarely examined. We selected the pioneer species Populus purdomii as a model plant to study the phoD-harboring bacterial communities in rhizosphere and bulk soils along a mountain glacier chronosequence. Our results showed that the bulk soils and rhizosphere host distinct phoD communities and diversity that differentially varied along the chronosequence, describing in detail the development and compositional turnover of the phoD community in the course of primary succession and determining the main environmental factors driving the development.

Project description:The growing concern about the effectiveness of reclamation strategies has motivated the evaluation of soil properties following reclamation. Recovery of belowground microbial community is important for reclamation success, however, the response of soil bacterial communities to reclamation has not been well understood. In this study, PCR-based 454 pyrosequencing was applied to compare bacterial communities in undisturbed soils with those in reclaimed soils using chronosequences ranging in time following reclamation from 1 to 20 year. Bacteria from the Proteobacteria, Chloroflexi, Actinobacteria, Acidobacteria, Planctomycetes and Bacteroidetes were abundant in all soils, while the composition of predominant phyla differed greatly across all sites. Long-term reclamation strongly affected microbial community structure and diversity. Initial effects of reclamation resulted in significant declines in bacterial diversity indices in younger reclaimed sites (1, 8-year-old) compared to the undisturbed site. However, bacterial diversity indices tended to be higher in older reclaimed sites (15, 20-year-old) as recovery time increased, and were more similar to predisturbance levels nearly 20 years after reclamation. Bacterial communities are highly responsive to soil physicochemical properties (pH, soil organic matter, Total N and P), in terms of both their diversity and community composition. Our results suggest that the response of soil microorganisms to reclamation is likely governed by soil characteristics and, indirectly, by the effects of vegetation restoration. Mixture sowing of gramineae and leguminosae herbage largely promoted soil geochemical conditions and bacterial diversity that recovered to those of undisturbed soil, representing an adequate solution for soil remediation and sustainable utilization for agriculture. These results confirm the positive impacts of reclamation and vegetation restoration on soil microbial diversity and suggest that the most important phase of microbial community recovery occurs between 15 and 20 years after reclamation.

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Dataset Information

Distribution and Succession Feature of Antibiotic Resistance Genes Along a Soil Development Chronosequence in Urumqi No.1 Glacier of China.

Publications

Distribution and Succession Feature of Antibiotic Resistance Genes Along a Soil Development Chronosequence in Urumqi No.1 Glacier of China.

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