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Salicornia europaea L. as an underutilized saline-tolerant plant inhabited by endophytic diazotrophs.

ABSTRACT: Despite the great interest in using halophyte Salicornia europaea L. as a crop in extreme saline habitats, little is known about the role played by associated endophytic bacteria in increasing tolerance of the host-plant to nutrient deficiency. Main objectives of this study were to investigate the community composition of diazotrophic endophytes of S. europaea grown under natural conditions, and determine the proportion of plant-growth promoting bacterial strains able to fix N₂. To quantify the abundance of diazotrophic bacterial endophytes in stems and roots of S. europaea, nifH gene and 16S rDNA copy numbers were assessed by quantitative real-time PCR, and characterized the taxonomic structure of cultivable bacteria based on selective medium for diazotrophs. The highest copy numbers of nifH and 16S rDNA were observed in the stems of plants growing at the test site characterized by lower salinity, and correlated with high N concentrations in plant tissues. The abundance of bacterial diazotrophs isolated from plant tissues ranged from 3.6 to 6.3 (log₁₀ of cfu per gram dry plant tissue) and varied in a site- and plant-organ manner. Proteobacteria dominated in plants growing in lower salinity while Actinobacteria prevailed in plants originating from higher salinity, what suggest better adaptation of this group of bacteria to extreme salinity. The results provide insights into new species of diazotrophs associated with halophytes that can be used to optimize strategies for selecting biostimulants useful in saline soils.

SUBMITTER: Hrynkiewicz K

PROVIDER: S-EPMC6630021 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Salicornia europaea L. as an underutilized saline-tolerant plant inhabited by endophytic diazotrophs.

Hrynkiewicz Katarzyna K Patz Sascha S Ruppel Silke S

Journal of advanced research 20190516

Despite the great interest in using halophyte Salicornia europaea L. as a crop in extreme saline habitats, little is known about the role played by associated endophytic bacteria in increasing tolerance of the host-plant to nutrient deficiency. Main objectives of this study were to investigate the community composition of diazotrophic endophytes of S. europaea grown under natural conditions, and determine the proportion of plant-growth promoting bacterial strains able to fix N ...[more]

PMID: 31341669

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Project description:We examined Salicornia europaea, a nonmycorrhizal halophyte associated with specific and unique endophytic bacteria and fungi. The microbial community structure was analyzed at two sites differing in salinization history (anthropogenic and naturally saline site), in contrasting seasons (spring and fall) and in two plant organs (shoots and roots) via 16S rRNA and internal transcribed spacer amplicon sequencing. We observed distinct communities at the two sites, and in shoots and roots, while the season was of no importance. The bacterial community was less diverse in shoot libraries than in roots, regardless of the site and season, whereas no significant differences were observed for the fungal community. Proteobacteria and Bacteroidetes dominated bacterial assemblages, and Ascomycetes were the most frequent fungi. A root core microbiome operational taxonomic unit belonging to the genus Marinimicrobium was identified. We detected a significant influence of the Salicornia bacterial community on the fungal one by means of cocorrespondence analysis. In addition, pathways and potential functions of the bacterial community in Salicornia europaea were inferred and discussed. We can conclude that bacterial and fungal microbiomes of S. europaea are determined by the origin of salinity at the sites. Bacterial communities seemed to influence fungal ones, but not the other way around, which takes us closer to understanding of interactions between the two microbial groups. In addition, the plant organs of the halophyte filter the microbial community composition.IMPORTANCE Endophytes are particularly fascinating because of their multifaceted lifestyle, i.e., they may exist as either free-living soil microbes or saprobic ones or pathogens. Endophytic communities of halophytes may be different than those in other plants because salinity acts as an environmental filter. At the same time, they may contribute to the host's adaptation to adverse environmental conditions, which may be of importance in agriculture.

Dataset Information

Salicornia europaea L. as an underutilized saline-tolerant plant inhabited by endophytic diazotrophs.

Publications

<i>Salicornia europaea</i> L. as an underutilized saline-tolerant plant inhabited by endophytic diazotrophs.

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