Dataset Information

Effects of Light Level and Nitrogen Supply on the Red Clover-Orobanche Minor Host-Parasite Interaction.

ABSTRACT: Infection by holoparasitic plants typically causes decreases in host mass, thought to be primarily as a result of resource abstraction. Inverse relationships have been noted between the number of Orobanche spp. parasites infecting a host and their mass, suggesting that the parasites compete for a shared resource pool, assumed to be recently fixed carbon (C). In clover, nitrogen (N) fixation requires a high proportion of daily photosynthate and represents a potential competitor for recently fixed C. We grew Trifolium pratense, either singly or parasitised by Orobanche minor, under high or low light levels, and with or without exogenous N supply. Low light and N deficiency led to decreased host biomass, while the damage caused by parasitism was proportionate to host mass. Parasitism caused reductions in host leaf mass, area, photosynthetic rates and shoot N concentration, but did not affect starch accumulation. Parasite mass as a proportion of system biomass was significantly higher when attached to plants grown at high light, which was attributed to higher photoassimilate supply, while the N supply had no effect. While both N limitation and parasitism caused reductions in host growth, little evidence of competition for C between N fixation and the parasites was noted.

SUBMITTER: Jokinen JI

PROVIDER: S-EPMC6631064 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Publications

Effects of Light Level and Nitrogen Supply on the Red Clover-<i>Orobanche Minor</i> Host-Parasite Interaction.

Jokinen Joel I JI Irving Louis J LJ

Plants (Basel, Switzerland) 20190531 6

Infection by holoparasitic plants typically causes decreases in host mass, thought to be primarily as a result of resource abstraction. Inverse relationships have been noted between the number of <i>Orobanche</i> spp. parasites infecting a host and their mass, suggesting that the parasites compete for a shared resource pool, assumed to be recently fixed carbon (C). In clover, nitrogen (N) fixation requires a high proportion of daily photosynthate and represents a potential competitor for recentl ...[more]

PMID: 31159192

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Project description:Broomrape, caused by the root holoparasite Orobanche cumana, is the main biotic constraint to sunflower oil production worldwide. By the time broomrape emerges, most of the metabolic imbalance has been produced by O. cumana to sunflower plants. UV-induced multicolor fluorescence imaging (MCFI) provides information on the fluorescence emitted by chlorophyll (Chl) a of plants in the spectral bands with peaks near 680 nm (red, F680) and 740 nm (far-red, F740). In this work MCFI was extensively applied to sunflowers, either healthy or parasitized plants, for the first time. The distribution of red and far-red fluorescence was analyzed in healthy sunflower grown in pots under greenhouse conditions. Fluorescence patterns were analyzed across the leaf surface and throughout the plant by comparing the first four leaf pairs (LPs) between the second and fifth week of growth. Similar fluorescence patterns, with a delay of 3 or 4 days between them, were obtained for LPs of healthy sunflower, showing that red and far-red fluorescence varied with the developmental stage of the leaf. The use of F680 and F740 as indicators of sunflower infection by O. cumana during underground development stages of the parasite was also evaluated under similar experimental conditions. Early increases in F680 and F740 as well as decreases in F680/F740 were detected upon infection by O. cumana. Significant differences between inoculated and control plants depended on the LP that was considered at any time. Measurements of Chl contents and final total Chl content supported the results of MCFI, but they were less sensitive in differentiating healthy from inoculated plants. Sunflower infection was confirmed by the presence of broomrape nodules in the roots at the end of the experiment. The potential of MCFI in the red and far-red region for an early detection of O. cumana infection in sunflower was revealed. This technique might have a particular interest for early phenotyping in sunflower breeding programs. To our knowledge, this is the first work where the effect of a parasitic plant in its host is analyzed by means of fluorescence imaging in the red and far-red spectral regions.

Dataset Information

Effects of Light Level and Nitrogen Supply on the Red Clover-Orobanche Minor Host-Parasite Interaction.

Publications

Effects of Light Level and Nitrogen Supply on the Red Clover-<i>Orobanche Minor</i> Host-Parasite Interaction.

Similar Datasets

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