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Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey.

ABSTRACT: A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies.

SUBMITTER: Albayrak H

PROVIDER: S-EPMC6631488 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey.

Albayrak Harun H Yazici Zafer Z Ozan Emre E Tamer Cuneyt C Abd El Wahed Ahmed A Wehner Stefanie S Ulrich Kristina K Weidmann Manfred M

Veterinary sciences 20190613 2

A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, an ...[more]

PMID: 31200585

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