Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins.

ABSTRACT: Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the need to maintain cell viability, a common problem in cell-based production. Previously, we demonstrated that colicins produced by CFPS based on crude Escherichia coli lysates are effective in eradicating antibiotic-tolerant bacteria known as persisters. However, we also found that some colicins have poor solubility or low cell-killing activity. In this study, we improved the solubility of colicin M from 16% to nearly 100% by producing it in chaperone-enriched E. coli extracts, resulting in enhanced cell-killing activit

SUBMITTER: Jin X 

PROVIDER: S-EPMC6632115 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature