Ontology highlight
ABSTRACT:
SUBMITTER: Phipson B
PROVIDER: S-EPMC6634992 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Phipson Belinda B Er Pei X PX Combes Alexander N AN Forbes Thomas A TA Howden Sara E SE Zappia Luke L Yen Hsan-Jan HJ Lawlor Kynan T KT Hale Lorna J LJ Sun Jane J Wolvetang Ernst E Takasato Minoru M Oshlack Alicia A Little Melissa H MH
Nature methods 20181220 1
The utility of human pluripotent stem cell-derived kidney organoids relies implicitly on the robustness and transferability of the protocol. Here we analyze the sources of transcriptional variation in a specific kidney organoid protocol. Although individual organoids within a differentiation batch showed strong transcriptional correlation, we noted significant variation between experimental batches, particularly in genes associated with temporal maturation. Single-cell profiling revealed shifts ...[more]