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Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing.

ABSTRACT: Whole genome bisulfite sequencing (WGBS), with its ability to interrogate methylation status at single CpG site resolution epigenome-wide, is a powerful technique for use in molecular experiments. Here, we aim to advance strategies for accurate and efficient WGBS for application in future large-scale epidemiological studies. We systematically compared the performance of three WGBS library preparation methods with low DNA input requirement (Swift Biosciences Accel-NGS, Illumina TruSeq and QIAGEN QIAseq) on two state-of-the-art sequencing platforms (Illumina NovaSeq and HiSeq X), and also assessed concordance between data generated by WGBS and methylation arrays. Swift achieved the highest proportion of CpG sites assayed and effective coverage at 26x?(P?

SUBMITTER: Zhou L

PROVIDER: S-EPMC6637168 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing.

Zhou Li L Ng Hong Kiat HK Drautz-Moses Daniela I DI Schuster Stephan C SC Beck Stephan S Kim Changhoon C Chambers John Campbell JC Loh Marie M

Scientific reports 20190717 1

Whole genome bisulfite sequencing (WGBS), with its ability to interrogate methylation status at single CpG site resolution epigenome-wide, is a powerful technique for use in molecular experiments. Here, we aim to advance strategies for accurate and efficient WGBS for application in future large-scale epidemiological studies. We systematically compared the performance of three WGBS library preparation methods with low DNA input requirement (Swift Biosciences Accel-NGS, Illumina TruSeq and QIAGEN ...[more]

PMID: 31316107

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