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Genetically Engineered Flagella Form Collagen-like Ordered Structures for Inducing Stem Cell Differentiation.

ABSTRACT: Bacteria use flagella, the protein nanofibers on their surface, as a molecular machine to swim. Flagella are polymerized from monomers, flagellins, which can display a peptide by genetic means. However, flagella as genetically modifiable nanofibers have not been used in building bone extracellular matrix-like structures for inducing stem cell differentiation in non-osteogenic medium. Here we discovered that interactions between Ca²⁺ ions and flagella (displaying a collagen-like peptide (GPP)₈ on every flagellin) resulted in ordered bundle-like structures, which were further mineralized with hydroxyapatite to form ordered fibrous matrix. The resultant matrix significantly induced the osteogenic differentiation of stem cells, much more efficiently than wild-type flagella and type I collagen. This work shows that flagella can be used as protein building blocks in generating biomimetic materials.

SUBMITTER: Li D

PROVIDER: S-EPMC6639685 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Genetically Engineered Flagella Form Collagen-like Ordered Structures for Inducing Stem Cell Differentiation.

Li Dong D Zhu Ye Y Yang Tao T Yang Mingying M Mao Chuanbin C

iScience 20190703

Bacteria use flagella, the protein nanofibers on their surface, as a molecular machine to swim. Flagella are polymerized from monomers, flagellins, which can display a peptide by genetic means. However, flagella as genetically modifiable nanofibers have not been used in building bone extracellular matrix-like structures for inducing stem cell differentiation in non-osteogenic medium. Here we discovered that interactions between Ca<sup>2+</sup> ions and flagella (displaying a collagen-like peptid ...[more]

PMID: 31323474

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