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Genetically Engineered Flagella Form Collagen-like Ordered Structures for Inducing Stem Cell Differentiation.


ABSTRACT: Bacteria use flagella, the protein nanofibers on their surface, as a molecular machine to swim. Flagella are polymerized from monomers, flagellins, which can display a peptide by genetic means. However, flagella as genetically modifiable nanofibers have not been used in building bone extracellular matrix-like structures for inducing stem cell differentiation in non-osteogenic medium. Here we discovered that interactions between Ca2+ ions and flagella (displaying a collagen-like peptide (GPP)8 on every flagellin) resulted in ordered bundle-like structures, which were further mineralized with hydroxyapatite to form ordered fibrous matrix. The resultant matrix significantly induced the osteogenic differentiation of stem cells, much more efficiently than wild-type flagella and type I collagen. This work shows that flagella can be used as protein building blocks in generating biomimetic materials.

SUBMITTER: Li D 

PROVIDER: S-EPMC6639685 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Genetically Engineered Flagella Form Collagen-like Ordered Structures for Inducing Stem Cell Differentiation.

Li Dong D   Zhu Ye Y   Yang Tao T   Yang Mingying M   Mao Chuanbin C  

iScience 20190703


Bacteria use flagella, the protein nanofibers on their surface, as a molecular machine to swim. Flagella are polymerized from monomers, flagellins, which can display a peptide by genetic means. However, flagella as genetically modifiable nanofibers have not been used in building bone extracellular matrix-like structures for inducing stem cell differentiation in non-osteogenic medium. Here we discovered that interactions between Ca<sup>2+</sup> ions and flagella (displaying a collagen-like peptid  ...[more]

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