Unknown

Dataset Information

0

Mass Spectrometry Measurement of Single Suspended Cells Using a Combined Cell Manipulation System and a Single-Probe Device.

ABSTRACT: Existing single cell mass spectrometry (SCMS) sampling platforms are largely designed to work only with immobilized cells and not the suspended cells isolated from patient samples. Here, we present a novel method that integrates a commercially available cell manipulation system commonly used for in vitro fertilization with the Single-probe SCMS sampling technology. The combined Single-probe SCMS/cell manipulating platform is capable of rapidly analyzing intracellular species in real time from a suspension leukemia cell line. A broad range of molecular species was detected, and species of interest were verified using tandem MS (MS/MS). Experimental results were analyzed utilizing statistical analyses such as principle component analysis (PCA) and  t-tests. The developed SCMS/cell manipulation system is a versatile tool to provide rapid single cell analysis of broad types of patient cell samples.

SUBMITTER: Standke SJ 

PROVIDER: S-EPMC6640145 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Mass Spectrometry Measurement of Single Suspended Cells Using a Combined Cell Manipulation System and a Single-Probe Device.

Standke Shawna J SJ   Colby Devon H DH   Bensen Ryan C RC   Burgett Anthony W G AWG   Yang Zhibo Z  

Analytical chemistry 20190118 3

Existing single cell mass spectrometry (SCMS) sampling platforms are largely designed to work only with immobilized cells and not the suspended cells isolated from patient samples. Here, we present a novel method that integrates a commercially available cell manipulation system commonly used for in vitro fertilization with the Single-probe SCMS sampling technology. The combined Single-probe SCMS/cell manipulating platform is capable of rapidly analyzing intracellular species in real time from a  ...[more]

Similar Datasets

Unknown Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells.
| S-EPMC6677261 | biostudies-literature
Unknown T-Probe: An Integrated Microscale Device for Online In Situ Single Cell Analysis and Metabolic Profiling Using Mass Spectrometry.
| S-EPMC6583895 | biostudies-literature
Unknown Using Dicationic Ion-Pairing Compounds To Enhance the Single Cell Mass Spectrometry Analysis Using the Single-Probe: A Microscale Sampling and Ionization Device.
| S-EPMC4935574 | biostudies-literature
Unknown Manipulation of Suspended Single Cells by Microfluidics and Optical Tweezers.
| S-EPMC2932633 | biostudies-literature
Unknown Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.
| S-EPMC6677389 | biostudies-literature
Unknown Metabolomic Fingerprints of Individual Algal Cells Using the Single-Probe Mass Spectrometry Technique.
| S-EPMC5936784 | biostudies-literature
Unknown Rapid Single Particle Atmospheric Solids Analysis Probe-Mass Spectrometry for Multimodal Analysis of Microplastics.
| S-EPMC9850409 | biostudies-literature
Unknown Integrating a generalized data analysis workflow with the Single-probe mass spectrometry experiment for single cell metabolomics.
| S-EPMC6579046 | biostudies-literature
Unknown Overcoming challenges in single particle inductively coupled plasma mass spectrometry measurement of silver nanoparticles.
| S-EPMC5693768 | biostudies-literature
Unknown Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix.
| S-EPMC4413513 | biostudies-literature