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P2 of rice stripe virus (RSV) interacts with OsSGS3 and is a silencing suppressor.

ABSTRACT: A rice cDNA library was screened by a galactosidase 4 (Gal4)-based yeast two-hybrid system with Rice stripe virus (RSV) p2 as bait. The results revealed that RSV p2 interacted with a rice protein exhibiting a high degree of identity with Arabidopsis thaliana suppressor of gene silencing 3 (AtSGS3). The interaction was confirmed by bimolecular fluorescence complementation assay. SGS3 has been shown to be involved in sense transgene-induced RNA silencing and in the biogenesis of trans-acting small interfering RNAs (ta-siRNAs), possibly functioning as a cofactor of RNA-dependent RNA polymerase 6 (RDR6). Given the intimate relationships between virus and RNA silencing, further experiments were conducted to show that p2 was a silencing suppressor. In addition, p2 enhanced the accumulation and pathogenicity of Potato virus X in Nicotiana benthamiana. Five genes that have been demonstrated to be targets of TAS3-derived ta-siRNAs were up-regulated in RSV-infected rice. The implications of these findings are discussed.

SUBMITTER: Du Z 

PROVIDER: S-EPMC6640460 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

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p2 of rice stripe virus (RSV) interacts with OsSGS3 and is a silencing suppressor.

Du Zhenguo Z   Xiao Donglai D   Wu Jianguo J   Jia Dongsheng D   Yuan Zhengjie Z   Liu Ying Y   Hu Liuyang L   Han Zhao Z   Wei Taiyun T   Lin Qiying Q   Wu Zujian Z   Xie Lianhui L  

Molecular plant pathology 20110427 8

A rice cDNA library was screened by a galactosidase 4 (Gal4)-based yeast two-hybrid system with Rice stripe virus (RSV) p2 as bait. The results revealed that RSV p2 interacted with a rice protein exhibiting a high degree of identity with Arabidopsis thaliana suppressor of gene silencing 3 (AtSGS3). The interaction was confirmed by bimolecular fluorescence complementation assay. SGS3 has been shown to be involved in sense transgene-induced RNA silencing and in the biogenesis of trans-acting small  ...[more]

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