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Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins.

ABSTRACT: This article describes a photochemical approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein bound to the surface in illuminated regions. Two different fluorescent proteins were patterned in registry with features of 400 nm in size over a 1 mm2 area. An example is given wherein an anti-carcinoembryonic antigen (anti-CEA) scFv antibody was patterned to direct the selective attachment of a human cancer cell line that express the CEA antigen. This method enables the preparation of surfaces with control over the density and activity of independently patterned proteins.

SUBMITTER: Zhou S

PROVIDER: S-EPMC6640637 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins.

Zhou Shengwang S Metcalf Kevin J KJ Bugga Pradeep P Grant Jennifer J Mrksich Milan M

ACS applied materials & interfaces 20181114 47

This article describes a photochemical approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein boun ...[more]

PMID: 30379516

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