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Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult Drosophila.

ABSTRACT: Olfactory associative learning in Drosophila is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the gamma subtype of Kenyon cells. mAChR-A inhibits odor responses and is localized in Kenyon cell dendrites. Moreover, mAChR-A knockdown impairs the learning-associated depression of odor responses in a mushroom body output neuron. Our results suggest that mAChR-A function in Kenyon cell dendrites is required for synaptic plasticity between Kenyon cells and their output neurons.

SUBMITTER: Bielopolski N

PROVIDER: S-EPMC6641838 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Inhibitory muscarinic acetylcholine receptors enhance aversive olfactory learning in adult <i>Drosophila</i>.

Bielopolski Noa N Amin Hoger H Apostolopoulou Anthi A AA Rozenfeld Eyal E Lerner Hadas H Huetteroth Wolf W Lin Andrew C AC Parnas Moshe M

eLife 20190619

Olfactory associative learning in <i>Drosophila</i> is mediated by synaptic plasticity between the Kenyon cells of the mushroom body and their output neurons. Both Kenyon cells and their inputs from projection neurons are cholinergic, yet little is known about the physiological function of muscarinic acetylcholine receptors in learning in adult flies. Here, we show that aversive olfactory learning in adult flies requires type A muscarinic acetylcholine receptors (mAChR-A), particularly in the ga ...[more]

PMID: 31215865

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