Unknown

Dataset Information

0

Reduced Graphene Oxide-Oligonucleotide Interfaces: Understanding Based on Electrochemical Oxidation of Guanines.


ABSTRACT: Investigation into the interactions between biomolecules DNA/RNA and carbon nanomaterials is very important for applications in bioassays and bioanalysis. Graphene and graphene oxide (GO) have been successfully adopted by exploiting the binding affinity difference between single-stranded oligonucleotides (ssDNA) and double-stranded oligonucleotides (dsDNA) to graphene sheets. In this work, we describe the electrochemical DNA oxidation with [Ru(bpy)3]2+ to understand the interaction between dsDNA (and corresponding ssDNA) and reduced graphene oxide (rGO). The electrochemical oxidation rate of guanine bases of ssDNA bound to rGO by electrochemically generated [Ru(bpy)3]3+ was much slower than those unbound to rGO. Our study revealed that ssDNA constrained on rGO was significantly protected from the electron transfer to [Ru(bpy)3]3+ because of ?,?-stacking interaction between nucleobases and rGO. On the other hand, the oxidation rates of 11-, 20-, and 27-mer dsDNA bound to rGO increased relative to those of dsDNA alone, demonstrating that the guanine bases of dsDNA on the interaction with rGO became more accessible to [Ru(bpy)3]3+. Our electrochemical data illustrated that dsDNA could be totally or partially dehybridized and bind to rGO to form ssDNA/rGO. Furthermore, absorption, circular dichroism spectra, and fluorescence measurements of ethidium bromide using ssDNA and dsDNA with rGO supported the dehybridization of dsDNA in the presence of rGO.

SUBMITTER: Tikum AF 

PROVIDER: S-EPMC6643540 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Reduced Graphene Oxide-Oligonucleotide Interfaces: Understanding Based on Electrochemical Oxidation of Guanines.

Tikum Anjong Florence AF   Ko Jeong Won JW   Kim Soojin S   Kim Jinheung J  

ACS omega 20181114 11


Investigation into the interactions between biomolecules DNA/RNA and carbon nanomaterials is very important for applications in bioassays and bioanalysis. Graphene and graphene oxide (GO) have been successfully adopted by exploiting the binding affinity difference between single-stranded oligonucleotides (ssDNA) and double-stranded oligonucleotides (dsDNA) to graphene sheets. In this work, we describe the electrochemical DNA oxidation with [Ru(bpy)<sub>3</sub>]<sup>2+</sup> to understand the int  ...[more]

Similar Datasets

| S-EPMC6753649 | biostudies-literature
| S-EPMC6915725 | biostudies-literature
| S-EPMC4440036 | biostudies-literature
| S-EPMC8695096 | biostudies-literature
| S-EPMC4193797 | biostudies-literature
| S-EPMC9140755 | biostudies-literature
| S-EPMC6202686 | biostudies-literature
| S-EPMC5245759 | biostudies-literature
| S-EPMC4604454 | biostudies-literature
| S-EPMC7610301 | biostudies-literature