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Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans.

ABSTRACT: We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans.

SUBMITTER: Silva-Garcia CG

PROVIDER: S-EPMC6643881 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Single-Copy Knock-In Loci for Defined Gene Expression in <i>Caenorhabditis elegans</i>.

Silva-García Carlos G CG Lanjuin Anne A Heintz Caroline C Dutta Sneha S Clark Nicole M NM Mair William B WB

G3 (Bethesda, Md.) 20190709 7

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the <i>Caenorhabditis elegans</i> genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes i ...[more]

PMID: 31064766

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