Esterase-Mediated Highly Fluorescent Gold Nanoclusters and Their Use in Ultrasensitive Detection of Mercury: Synthetic and Mechanistic Aspects.

ABSTRACT: The fast, accurate, and ultrasensitive detection of toxic mercury in real water samples is still challenging without the use of expensive sophisticated instruments. Herein, highly fluorescent gold nanoclusters (AuNCs) were synthesized using a newer protein templet, esterase (EST). The EST-AuNCs consisted of ∼25 Au atoms in the nanocluster having ∼2 nm size. EST-AuNCs were found to be highly stable in aqueous buffer with a wide range of pH (pH 4-12) and were also stable in powdered form. The fluorescence quantum yield of EST-AuNCs in deionized water was 6.2% which had increased to 7.8% upon the addition of 1 M NaCl (an increase of 23%). The EST-AuNCs selectively sense the toxic Hg²⁺ ions with higher sensitivity (limit of detection; 0.88 nM) with the linear range 1-30 nM. The test

SUBMITTER: Thakur NS 

PROVIDER: S-EPMC6643912 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature