Dataset Information

Influence of Metal Ions on the Immobilization of ?-Glucosidase Through Protein-Inorganic Hybrids.

ABSTRACT: Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of ?-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temperature stability compared to free form. Thermal stability of hybrid improved up to 26-fold at 60 °C. After 10 cycles of reuse, immobilized enzyme retained 93.8% of residual activity. These results suggested that metal ions played a significant role in the enzyme immobilization as a protein-inorganic hybrid. Overall, this strategy can be potentially applied to enhance the properties of enzymes though effective encapsulation for the broad biotechnological applications.

SUBMITTER: Patel SKS

PROVIDER: S-EPMC6646435 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids.

Patel Sanjay K S SKS Gupta Rahul K RK Kumar Virendra V Mardina Primata P Lestari Rowina R Kalia Vipin C VC Choi Myung-Seok MS Lee Jung-Kul JK

Indian journal of microbiology 20190328 3

Immobilization of enzymes through metal-based system is demonstrated as a promising approach to enhance its properties. In this study, the influence of metals ions, including copper, cobalt and zinc (Zn) on the immobilization of β-glucosidase (BGL) through the synthesis of protein-inorganic hybrid was evaluated at 4 °C. Among these metal ions-based hybrids, Zn showed the highest encapsulation yield and relative activity of 87.5 and 207%, respectively. Immobilized BGL exhibited higher pH and temp ...[more]

PMID: 31388216

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Project description:In this study, we used solvent assisted mechano-synthesis strategies to form multifunctional organic-inorganic nanocomposites capable of removing both organic and inorganic contaminants. A zeolite X (Ze) and activated carbon (AC) composite was synthesized via state-of-the-art mechanical mixing in the presence of few drops of water to form Ze/AC. The second composite (Ze/L/AC) was synthesized in a similar fashion, however this composite had the addition of disodium terephthalate as a linker. Both materials, Ze/AC and Ze/L/AC, were characterized using scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), Powdered X-ray diffraction (P-XRD), Fourier-transform infrared spectrometry (FTIR), Accelerated Surface Area and Porosimetry System (ASAP), and thermal gravimetric analysis (TGA). The SEM-EDS displayed the surface structure and composition of each material. The sodium, oxygen and carbon contents increased after linker connected Ze and AC. The P-XRD confirmed the crystallinity of each material as well as the composites, while FTIR indicated the function groups (C=C, O-H) in Ze/L/AC. The contaminant adsorption experiments investigated the effects of pH, temperature, and ionic strength on the adsorption of methylene blue (MB) and Co(II) for each material. In MB adsorption, the first-order reaction rate of Ze/L/AC (0.02 h-1) was double that of Ze/AC (0.01 h-1). The reaction rate of Ze/L/AC (4.8 h-1) was also extraordinarily higher than that of Ze/AC (0.6 h-1) in the adsorption of Co(II). Ze/L/AC composite achieved a maximum adsorption capacity of 44.8 mg/g for MB and 66.6 mg/g for Co(II) ions. The MB adsorption of Ze/AC and Ze/L/AC was best fit in Freundlich model with R2 of 0.96 and 0.97, respectively, which indicated the multilayer adsorption. In the Co(II) adsorption, the data was highly fit in Langmuir model with R2 of 0.94 and 0.92 which indicated the monolayer adsorption. These results indicated both materials exhibited chemisorption. The activation energy of Ze/L/AC in MB adsorption (34.9 kJ mol-1) was higher than that of Ze/L/AC in Co (II) adsorption (26 kJ mol-1).

Dataset Information

Influence of Metal Ions on the Immobilization of ?-Glucosidase Through Protein-Inorganic Hybrids.

Publications

Influence of Metal Ions on the Immobilization of β-Glucosidase Through Protein-Inorganic Hybrids.

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