Dataset Information

Foliar Application of Iron Fortified Bacteriosiderophore Improves Growth and Grain Fe Concentration in Wheat and Soybean.

ABSTRACT: Iron (Fe) is one of the key micronutrients essential for plant growth, yield and quality. Wheat (Triticum aestivum) and soybean (Glycine max) are important food crops but have relatively low Fe content in grains/seeds. Foliar application of Fe-invigorated bacteriosiderophore might increase Fe content in grain as well as improve overall plant growth. From a preliminary experiment conducted on soybean using 20 bacterial strains, Arthrobacter sp. (low siderophore producing) and Lysinibacillus fusiformis (high siderophore producing) were selected based on amount of siderophore produced and response of plants. This result was validated on field grown soybean and wheat crops by applying bacteriosiderophore with or without Fe on foliage. Siderophore was applied at flowering stage in both crops and observations were recorded on the sixth day after foliar spray. Significantly higher shoot biomass, area of leaves or flag leaf and tissue Fe concentration was recorded by siderophore produced by L. fusiformis with Fe as compared to Arthrobacter sp. In comparison to control (water), application of Fe fortified bacterial siderophore resulted not only in increased grain yield by 45% and 28% in wheat and soybean, respectively but also enhanced Fe concentration in grains by 1.7-fold in soybean to 2.0-fold in wheat. Partitioning of Fe in grain was higher in wheat as compared to soybean after foliar spray. Thus, we reported for the first time that bacteriosiderophore with added Fe as foliar application could be an economical and targeted agronomic approach towards Fe fortification in crop plants.

SUBMITTER: Sharma S

PROVIDER: S-EPMC6646439 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Foliar Application of Iron Fortified Bacteriosiderophore Improves Growth and Grain Fe Concentration in Wheat and Soybean.

Sharma Sandeep S Chandra Subhash S Kumar Arun A Bindraban Prem P Saxena Anil Kumar AK Pande Veena V Pandey Renu R

Indian journal of microbiology 20190608 3

Iron (Fe) is one of the key micronutrients essential for plant growth, yield and quality. Wheat (Triticum aestivum) and soybean (Glycine max) are important food crops but have relatively low Fe content in grains/seeds. Foliar application of Fe-invigorated bacteriosiderophore might increase Fe content in grain as well as improve overall plant growth. From a preliminary experiment conducted on soybean using 20 bacterial strains, Arthrobacter sp. (low siderophore producing) and ...[more]

PMID: 31388212

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Project description:Wheat is an important dietary source of zinc (Zn) and other mineral elements in many countries. Dietary Zn deficiency is widespread, especially in developing countries, and breeding (genetic biofortification) through the HarvestPlus programme has recently started to deliver new wheat varieties to help alleviate this problem in South Asia. To better understand the potential of wheat to alleviate dietary Zn deficiency, this study aimed to characterise the baseline effects of genotype (G), site (E), and genotype by site interactions (GxE) on grain Zn concentration under a wide range of soil conditions in India. Field experiments were conducted on a diverse panel of 36 Indian-adapted wheat genotypes, grown on a range of soil types (pH range 4.5-9.5), in 2013-14 (five sites) and 2014-15 (six sites). Grain samples were analysed using inductively coupled plasma-mass spectrometry (ICP-MS). The mean grain Zn concentration of the genotypes ranged from 24.9-34.8 mg kg-1, averaged across site and year. Genotype and site effects were associated with 10% and 6% of the overall variation in grain Zn concentration, respectively. Whilst G x E interaction effects were evident across the panel, some genotypes had consistent rankings between sites and years. Grain Zn concentration correlated positively with grain concentrations of iron (Fe), sulphur (S), and eight other elements, but did not correlate negatively with grain yield, i.e. no yield dilution was observed. Despite a relatively small contribution of genotype to the overall variation in grain Zn concentration, due to experiments being conducted across many contrasting sites and two years, our data are consistent with reports that biofortifying wheat through breeding is likely to be effective at scale given that some genotypes performed consistently across diverse soil types. Notably, all soils in this study were probably Zn deficient and interactions between wheat genotypes and soil Zn availability/management (e.g. the use of Zn-containing fertilisers) need to be better-understood to improve Zn supply in food systems.

Project description:Genomic regions responsible for accumulation of grain iron concentration (Fe), grain zinc concentration (Zn), grain protein content (PC) and thousand kernel weight (TKW) were investigated in 286 recombinant inbred lines (RILs) derived from a cross between an old Indian wheat variety WH542 and a synthetic derivative (Triticum dicoccon PI94624/Aegilops squarrosa [409]//BCN). RILs were grown in six environments and evaluated for Fe, Zn, PC, and TKW. The population showed the continuous distribution for all the four traits, that for pooled Fe and PC was near normal, whereas, for pooled Zn, RILs exhibited positively skewed distribution. A genetic map spanning 2155.3cM was constructed using microsatellite markers covering the 21 chromosomes and used for QTL analysis. 16 quantitative trait loci (QTL) were identified in this study. Four QTLs (QGFe.iari-2A, QGFe.iari-5A, QGFe.iari-7A and QGFe.iari-7B) for Fe, five QTLs (QGZn.iari-2A, QGZn.iari-4A, QGZn.iari-5A, QGZn.iari-7A and QGZn.iari-7B) for Zn, two QTLs (QGpc.iari-2A and QGpc.iari-3A) for PC, and five QTLs (QTkw.iari-1A, QTkw.iari-2A, QTkw.iari-2B, QTkw.iari-5B and QTkw.iari-7A) for TKW were identified. The QTLs together explained 20.0%, 32.0%, 24.1% and 32.3% phenotypic variation, respectively, for Fe, Zn, PC and TKW. QGpc.iari-2A was consistently expressed in all the six environments, whereas, QGFe.iari-7B and QGZn.iari-2A were identified in two environments each apart from pooled mean. QTkw.iari-2A and QTkw.iari-7A, respectively, were identified in four and three environments apart from pooled mean. A common region in the interval of Xgwm359-Xwmc407 on chromosome 2A was associated with Fe, Zn, and PC. One more QTL for TKW was identified on chromosome 2A but in a different chromosomal region (Xgwm382-Xgwm359). Two more regions on 5A (Xgwm126-Xgwm595) and 7A (Xbarc49-Xwmc525) were found to be associated with both Fe and Zn. A QTL for TKW was identified (Xwmc525-Xbarc222) in a different chromosomal region on the same chromosome (7A). This reflects at least a partly common genetic basis for the four traits. It is concluded that fine mapping of the regions of the three chromosomes of A genome involved in determining the accumulation of Fe, Zn, PC, and TKW in this mapping population may be rewarding.

Dataset Information

Foliar Application of Iron Fortified Bacteriosiderophore Improves Growth and Grain Fe Concentration in Wheat and Soybean.

Publications

Foliar Application of Iron Fortified Bacteriosiderophore Improves Growth and Grain Fe Concentration in Wheat and Soybean.

Similar Datasets

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