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Fine mapping and identification of the fuzzless gene GaFzl in DPL972 (Gossypium arboreum).


ABSTRACT: KEY MESSAGE:The fuzzless gene GaFzl was fine mapped to a 70-kb region containing a GIR1 gene, Cotton_A_11941, responsible for the fuzzless trait in Gossypium arboreum DPL972. Cotton fiber is the most important natural textile resource. The fuzzless mutant DPL972 (Gossypium arboreum) provides a useful germplasm resource to explore the molecular mechanism underlying fiber and fuzz initiation and development. In our previous research, the fuzzless gene in DPL972 was identified as a single dominant gene and named GaFzl. In the present study, we fine mapped this gene using F2 and BC1 populations. By combining traditional map-based cloning and next-generation sequencing, we mapped GaFzl to a 70-kb region containing seven annotated genes. RNA-Sequencing and re-sequencing analysis narrowed these candidates to two differentially expressed genes, Cotton_A_11941 and Cotton_A_11942. Sequence alignment uncovered no variation in coding or promoter regions of Cotton_A_11942 between DPL971 and DPL972, whereas two single-base mutations in the promoter region and a TTG insertion in the coding region were detected in Cotton_A_11941 in DPL972. Cotton_A_11941 encoding a homologous gene of GIR1 (GLABRA2-interacting repressor) in Arabidopsis thaliana is thus the candidate gene most likely responsible for the fuzzless trait in DPL972. Our findings should lead to a better understanding of cotton fuzz formation, thereby accelerating marker-assisted selection during cotton breeding.

SUBMITTER: Feng X 

PROVIDER: S-EPMC6647196 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Fine mapping and identification of the fuzzless gene GaFzl in DPL972 (Gossypium arboreum).

Feng Xiaoxu X   Cheng Hailiang H   Zuo Dongyun D   Zhang Youping Y   Wang Qiaolian Q   Liu Ke K   Ashraf Javaria J   Yang Qiuhong Q   Li Simin S   Chen Xiaoqin X   Song Guoli G  

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 20190402 8


<h4>Key message</h4>The fuzzless gene GaFzl was fine mapped to a 70-kb region containing a GIR1 gene, Cotton_A_11941, responsible for the fuzzless trait in Gossypium arboreum DPL972. Cotton fiber is the most important natural textile resource. The fuzzless mutant DPL972 (Gossypium arboreum) provides a useful germplasm resource to explore the molecular mechanism underlying fiber and fuzz initiation and development. In our previous research, the fuzzless gene in DPL972 was identified as a single d  ...[more]

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