Dataset Information

Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs.

ABSTRACT:

Background

Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.

Methods

We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m²) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) adipocyte-derived extracellular vesicle microRNAs in serum; (3) the role of visceral adipose tissue extracellular vesicle in regulation of cholesterol efflux and cholesterol efflux gene expression in THP-1 macrophages in vitro.

Results

Efflux capacity was significantly associated with HDL (r = 0.30, p = 0.01) and LDL (r = 0.33, p = 0.005) particle size. Multivariate-analysis identified six microRNAs associated (p < 0.05) with cholesterol efflux capacity: miR-3129-5p (Beta = 0.695), miR-20b (0.430), miR9-5p (0.111), miR-320d (- 0.190), miR301a-5p (0.042), miR-155-5p (0.004). In response to increasing concentrations (1 μg/mL vs. 3 μg/mL) of VAT extracellular vesicle, cholesterol efflux (66% ± 10% vs. 49% ± 2%; p < 0.01) and expression of ABCA1 (FC = 1.9 ± 0.8 vs 0.5 ± 0.2; p < 0.001), CD36 (0.7 ± 0.4 vs. 2.1 ± 0.8, p = 0.02), CYP27A1 (1.4 ± 0.4 vs. 0.9 ± 0.5; p < 0.05), and LXRA (1.8 ± 1.1 vs. 0.5 ± 0.2; p < 0.05) was altered in THP-1 cells in vitro.

Conclusion

Adipocyte-derived extracellular vesicle microRNAs may, in part, be involved macrophage cholesterol efflux regulation.

SUBMITTER: Barberio MD

PROVIDER: S-EPMC6647309 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs.

Barberio Matthew D MD Kasselman Lora J LJ Playford Martin P MP Epstein Samuel B SB Renna Heather A HA Goldberg Madeleine M DeLeon Joshua J Voloshyna Iryna I Barlev Ashley A Salama Michael M Ferrante Sarah C SC Nadler Evan P EP Mehta Nehal N Reiss Allison B AB Freishtat Robert J RJ

Journal of translational medicine 20190722 1

<h4>Background</h4>Macrophage cholesterol efflux capacity has been identified as a predictor for cardiovascular disease. We assessed the relationship between adipocyte-derived extracellular vesicle microRNAs and macrophage cholesterol efflux capacity.<h4>Methods</h4>We assessed an adolescent cohort (n = 93, Age, median (IQR) = 17 (3) year, Female = 71, Male = 22) throughout the BMI continuum (BMI = 45.2 (13.2) kg/m<sup>2</sup>) for: (1) cholesterol efflux capacity and lipoprotein profiles; (2) a ...[more]

PMID: 31331347

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Dataset Information

Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs.

Background

Methods

Results

Conclusion

Publications

Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs.

Similar Datasets

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