Dataset Information

Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.

ABSTRACT:

Impact statement

Successful clinical tissue engineering requires functional fidelity of the cultured cell to its in vivo counterpart, but this has been elusive in renal tissue engineering. Typically, renal proximal tubule cells in culture have a flattened morphology and do not express key transporters essential to their function. In this article, we show for the first time that in vitro substrate mechanical properties dictate differentiation of cultured renal proximal tubule cells. Remarkably, this effect was only discernable after 4 weeks in culture, longer than usually reported for this cell type. These results demonstrate a new tunable parameter to optimize cell differentiation in renal tissue engineering.

SUBMITTER: Love HD

PROVIDER: S-EPMC6648172 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.

Love Harold D HD Ao Mingfang M Jorgensen Seiver S Swearingen Lindsey L Ferrell Nicholas N Evans Rachel R Gewin Leslie L Harris Raymond C RC Zent Roy R Roy Shuvo S Fissell William H WH

Tissue engineering. Part A 20190614 13-14

<h4>Impact statement</h4>Successful clinical tissue engineering requires functional fidelity of the cultured cell to its <i>in vivo</i> counterpart, but this has been elusive in renal tissue engineering. Typically, renal proximal tubule cells in culture have a flattened morphology and do not express key transporters essential to their function. In this article, we show for the first time that <i>in vitro</i> substrate mechanical properties dictate differentiation of cultured renal proximal tubul ...[more]

PMID: 30484388

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Dataset Information

Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.

Impact statement

Publications

Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.

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