Design and Applications of a Fluorescent Labeling Technique for Lipid and Surfactant Preformed Vesicles.

ABSTRACT: Amphiphilic molecules such as surfactants, lipids, and block copolymers can be assembled into bilayers and form vesicles. Fluorescent membrane labeling methods require the use of dye molecules that can be inserted into the bilayers at different stages of synthesis. To our knowledge, there is no generalized method for labeling preformed vesicles. Herein, we develop a versatile protocol that is suitable to both surfactant and lipid preformed vesicles and requires no separation or purification steps. On the basis of the lipophilic carbocyanine green dye PKH67, the methodology is assessed on zwitterionic phosphatidylcholine vesicles. To demonstrate its versatility, it is applied to dispersions of anionic or cationic vesicles, such as a drug administrated to premature infants with respiratory d

SUBMITTER: Mousseau F 

PROVIDER: S-EPMC6648494 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature