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Femtosecond Thin-Film Laser Amplifiers Using Chirped Gratings.

ABSTRACT: Ultrafast injection-locked amplification is achieved by sending femtosecond supercontinuum pulses into a polymeric thin film coated on a distributed feedback (DFB) microcavity consisting of chirped gratings. The spatial variation of the grating period led to the resonance of the DFB microcavity at different wavelengths for injection at different locations. This enables convenient and continuous tuning of the amplification spectrum by displacing the grating structures. The large area of the grating structures enabled large tuning range. The amplified spectrum can be continuously tuned from 545 to 580 nm through sliding the grating structures by about 3.5 mm. Sub-1 ps lifetime has been measured for the amplification process with a net amplification factor as large as 33. Injection locking enabled high-quality control of the divergence and transverse mode of the output laser beam.

SUBMITTER: Wang M

PROVIDER: S-EPMC6648656 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Femtosecond Thin-Film Laser Amplifiers Using Chirped Gratings.

Wang Meng M Zhang Xinping X

ACS omega 20190501 5

Ultrafast injection-locked amplification is achieved by sending femtosecond supercontinuum pulses into a polymeric thin film coated on a distributed feedback (DFB) microcavity consisting of chirped gratings. The spatial variation of the grating period led to the resonance of the DFB microcavity at different wavelengths for injection at different locations. This enables convenient and continuous tuning of the amplification spectrum by displacing the grating structures. The large area of the grati ...[more]

PMID: 31459886

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Project description:Airway organoids derived from adult murine epithelial cells represent a complex 3D in vitro system mimicking the airway epithelial tissue’s native cell composition and physiological properties. In combination with a precise damage induction via femtosecond laser-based nanosurgery, this model allows for the examination of intra- and intercellular dynamics in the course of repair processes with a high spatio-temporal resolution, which can hardly be reached using in vivo approaches. For characterization of the organoids’ response to single or multiple-cell ablation, we first analyzed overall organoid survival and found that airway organoids were capable of efficiently repairing damage induced by femtosecond laser-based ablation of a single to ten cells within 24 h. An EdU staining assay further revealed a steady proliferative potential of airway organoid cells. Especially in the case of ablation of five cells, proliferation was enhanced within the first four hours upon damage induction, whereas ablation of ten cells was followed by a slight decrease in proliferation within this time frame. Analyzing individual trajectories of single cells within airway organoids, we found an increased migratory behavior in cells within close proximity to the ablation site following the ablation of ten, but not five cells. Bulk RNA sequencing and subsequent enrichment analysis revealed the differential expression of sets of genes involved in the regulation of epithelial repair, distinct signaling pathway activities such as Notch signaling, as well as cell migration after laser-based ablation. Together, our findings demonstrate that organoid repair upon ablation of ten cells involves the same key processes by which native airway epithelial wound healing is regulated. This marks the herein presented in vitro damage model suitable to study repair processes following acute airway injury, thereby posing a novel approach to gain insights into the mechanisms driving epithelial repair on a single-cell level.

Dataset Information

Femtosecond Thin-Film Laser Amplifiers Using Chirped Gratings.

Publications

Femtosecond Thin-Film Laser Amplifiers Using Chirped Gratings.

Similar Datasets

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