Dataset Information

A Redox-Switchable Colorimetric Probe for "Naked-Eye" Detection of Hypochlorous Acid and Glutathione.

ABSTRACT: We report the development of a new colorimetric probe (L-ol) for investigations of the redox process regulated by hypochlorous acid (HOCl) and glutathione (GSH). The HOCl/GSH redox-switching cycle process was investigated in detail by UV-vis absorption spectroscopy, colorimetric analysis assay and high-resolution mass spectrometry (HRMS). The switchable absorbance responses were attributed to the HOCl-induced oxidation of the p-methoxyphenol unit to the benzoquinone derivative (L-one) and sequential reduction of L-one to hydroquinone (L-ol') by GSH. In phosphate-buffered saline (PBS) buffer, the absorbance of L-ol at 619 nm underwent a remarkable bathochromic-shift, accompanied by a color change from pale yellow to blue in the presence of HOCl. With further addition of GSH, the absorbance of L-one exclusively recovered to the original level. Meanwhile, the blue-colored solution returned to the naive pale yellow color in the presence of GSH. The detection limits for HOCl and GSH were calculated to be 6.3 and 96 nM according to the IUPAC criteria. Furthermore, L-ol-loaded chromatography plates have been prepared and successfully applied to visualize and quantitatively analyze HOCl in several natural waters.

SUBMITTER: Han Q

PROVIDER: S-EPMC6651190 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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A Redox-Switchable Colorimetric Probe for "Naked-Eye" Detection of Hypochlorous Acid and Glutathione.

Han Qian Q Zhou Fang F Wang Yue Y Feng Huan H Meng Qingtao Q Zhang Zhiqiang Z Zhang Run R

Molecules (Basel, Switzerland) 20190704 13

We report the development of a new colorimetric probe (L-ol) for investigations of the redox process regulated by hypochlorous acid (HOCl) and glutathione (GSH). The HOCl/GSH redox-switching cycle process was investigated in detail by UV-vis absorption spectroscopy, colorimetric analysis assay and high-resolution mass spectrometry (HRMS). The switchable absorbance responses were attributed to the HOCl-induced oxidation of the p-methoxyphenol unit to the benzoquinone derivative ( ...[more]

PMID: 31277409

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Project description:Arsenic (As) as a metal ion has long-term toxicity and its presence in water poses a serious threat to the environment and human health. So, rapid and accurate recognition of traces of As is of particular importance in environmental and natural resources. In this study, a fast and sensitive colorimetric method was developed using silver nano prisms (Ag NPrs), cysteine-capped Ag NPrs, and methionine-capped Ag NPrs for accurate detection of arsenic-based on transforming the morphology of silver nanoparticles (AgNPs). The generated Ag atoms from the redox reaction of silver nitrate and As(iii) were deposited on the surface of Ag NPrs and their morphology changed to a circle. The morphological changes resulted in a change in the color of the nanoparticles from blue to purple, which was detectable by the naked eye. The rate of change was proportional to the concentration of arsenic. The changes were also confirmed using UV-Vis absorption spectra and showed a linear relationship between the change in adsorption peak and the concentration of arsenic in the range of 0.0005 to 1 ppm with a lower limit of quantification (LLOQ) of 0.0005 ppm. The proposed probes were successfully used to determine the amount of As(iii) in human urine samples. In addition, modified microfluidic substrates were fabricated with Ag NPrs, Cys-capped Ag NPrs, and methionine-capped Ag NPrs nanoparticles that are capable of arsenic detection in the long-time and can be used in the development of on-site As(iii) detection kits. In addition, silver nanowires (AgNWs) were used as a probe to detect arsenic, but good results were not obtained in human urine specimens and paper microfluidic platforms. In this study, for the first time, AgNPs were developed for optical colorimetric detection of arsenic using paper-based microfluidics. Ag NPrs performed best in both optical and colorimetric techniques. Therefore, they can be a promising option for the development of sensitive, inexpensive, and portable tools in the environmental and biomedical diagnosis of As(iii).

Dataset Information

A Redox-Switchable Colorimetric Probe for "Naked-Eye" Detection of Hypochlorous Acid and Glutathione.

Publications

A Redox-Switchable Colorimetric Probe for "Naked-Eye" Detection of Hypochlorous Acid and Glutathione.

Similar Datasets

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