A Redox-Switchable Colorimetric Probe for "Naked-Eye" Detection of Hypochlorous Acid and Glutathione.

ABSTRACT: We report the development of a new colorimetric probe (L-ol) for investigations of the redox process regulated by hypochlorous acid (HOCl) and glutathione (GSH). The HOCl/GSH redox-switching cycle process was investigated in detail by UV-vis absorption spectroscopy, colorimetric analysis assay and high-resolution mass spectrometry (HRMS). The switchable absorbance responses were attributed to the HOCl-induced oxidation of the p-methoxyphenol unit to the benzoquinone derivative (L-one) and sequential reduction of L-one to hydroquinone (L-ol') by GSH. In phosphate-buffered saline (PBS) buffer, the absorbance of L-ol at 619 nm underwent a remarkable bathochromic-shift, accompanied by a color change from pale yellow to blue in the presence of HOCl. Wit

SUBMITTER: Han Q 

PROVIDER: S-EPMC6651190 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature