Dataset Information

Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for Revealing Regulatory Pathways Associated With Quercetin-Induced Apoptosis in HCT-116 Cells.

ABSTRACT: Quercetin (QUE) is a bioactive component that belongs to the natural flavonoids group, and recent researchers found that it could prevent colorectal cancer (CRC). However, the exact mechanism by which QUE exerts its anti-tumor effects in CRC remains unclear. In this study, MTS assay and flow cytometry were used to detect the anti-tumor effects of QUE on HCT-116 cells. The results showed that QUE could inhibit the proliferation and induce apoptosis of HCT-116 cells. Furthermore, whole transcriptome sequencing was employed to establish the microRNA (miRNA), long non-coding RNA (lncRNA), circular RNA (circRNA), and mRNA profiles. A total of 240 differentially expressed lncRNAs (DElncRNAs), 131 circRNAs (DEcircRNAs), 83 miRNAs (DEmiRNAs), and 1415 mRNAs (DEmRNAs) were identified in the QUE-treated HCT-116 cells compared to the untreated HCT-116 cells. Then, quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression of selected circRNAs, miRNAs, lncRNAs, and mRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to further investigate RNAs' biological functions and potential mechanisms. Based on the theory of competing endogenous RNA (ceRNA), the circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory networks were constructed to illustrate the regulatory relationship between non-coding RNA (ncRNA) and mRNA. Our results provided novel information about the molecular basis of QUE in treating CRC. Our findings indicated that deep RNA sequencing analysis of mRNA and ncRNAs was a promising approach to research anticancer mechanisms.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC6651514 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for Revealing Regulatory Pathways Associated With Quercetin-Induced Apoptosis in HCT-116 Cells.

Zhang Zheyu Z Li Bin B Xu Panpan P Yang Bo B

Frontiers in pharmacology 20190717

Quercetin (QUE) is a bioactive component that belongs to the natural flavonoids group, and recent researchers found that it could prevent colorectal cancer (CRC). However, the exact mechanism by which QUE exerts its anti-tumor effects in CRC remains unclear. In this study, MTS assay and flow cytometry were used to detect the anti-tumor effects of QUE on HCT-116 cells. The results showed that QUE could inhibit the proliferation and induce apoptosis of HCT-116 cells. Furthermore, whole transcripto ...[more]

PMID: 31379573

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Project description:Curcumin is the main component of the Chinese herbal plant turmeric, which has been demonstrated to possess antitumor and other pharmacological properties. The aim of the present study was to investigate the effects of curcumin on the viability, migration and apoptosis of human colorectal carcinoma HCT?116 cells, and to explore the underlying molecular mechanisms. In addition, it was investigated whether the antitumor effect of curcumin on HCT?116 cells could match that of the chemotherapeutic drug 5?fluorouracil (5?FU). HCT?116 cells were treated with curcumin (10, 20 and 30 µM) and 5?FU (500 µM), and cell viability and proliferation were detected by Cell Counting Kit?8 and colony formation assays, respectively. The migration and invasion of treated cells were determined using Transwell and carboxyfluorescein succinimidyl amino ester fluorescent labeling assays. Cell cycle distribution and apoptosis rates were detected by flow cytometry. Furthermore, cell morphology changes associated with apoptosis were observed by fluorescence microscopy with acridine orange/ethidium bromide dual staining. To investigate the possible underlying molecular mechanisms, the gene and protein levels of Fas, Fas?associated via death domain (FADD), caspase?8, caspase?3, matrix metalloproteinase (MMP)?9, nuclear factor (NF)??B, E?cadherin and claudin?3 were detected using quantitative PCR analysis, zymography and western blotting. The results revealed that curcumin markedly inhibited the viability and proliferation of HCT?116 cells in a dose? and time?dependent manner. The migration, aggregation and invasion of HCT?116 cells into the lungs of mice were decreased by curcumin treatment in a dose?dependent manner. S?phase arrest and gradually increased apoptotic rates of HCT?116 cells were observed with increasing curcumin concentrations. Additionally, the mRNA and protein levels of apoptosis?associated proteins (Fas, FADD, caspase?8 and caspase?3) and E?cadherin in HCT?116 cells were upregulated following treatment with curcumin in a dose?dependent manner. By contrast, the expression of migration?associated proteins, including MMP?9, NF??B and claudin?3, was downregulated with increasing curcumin concentrations. These data suggested that the inhibitory effect of curcumin on HCT?116 cells may match that of 5?FU. Therefore, curcumin induced cell apoptosis and inhibited tumor cell metastasis by regulating the NF??B signaling pathway, and its therapeutic effect may be comparable to that of 5?FU.

Dataset Information

Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for Revealing Regulatory Pathways Associated With Quercetin-Induced Apoptosis in HCT-116 Cells.

Publications

Integrated Whole Transcriptome Profiling and Bioinformatics Analysis for Revealing Regulatory Pathways Associated With Quercetin-Induced Apoptosis in HCT-116 Cells.

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