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LAMP real-time turbidity detection for fowl adenovirus.

ABSTRACT: BACKGROUND:Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established. RESULTS:The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/?L of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR. CONCLUSION:The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.

SUBMITTER: Yuan XY

PROVIDER: S-EPMC6651964 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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LAMP real-time turbidity detection for fowl adenovirus.

Yuan Xiao-Yuan XY Wang You-Ling YL Meng Kai K Zhang Yu-Xia YX Xu Huai-Ying HY Ai Wu W

BMC veterinary research 20190723 1

<h4>Background</h4>Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.<h4>Results</h4>The established assay was specific to FAdV-4, and the lower ...[more]

PMID: 31337397

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