Ontology highlight
ABSTRACT:
SUBMITTER: Goldman AR
PROVIDER: S-EPMC6653605 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Goldman Aaron R AR Beer Lynn A LA Tang Hsin-Yao HY Hembach Peter P Zayas-Bazan Delaine D Speicher David W DW
Current protocols in protein science 20190610 1
This article describes processing of protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed-phase nanocapillary ultra-high-pressure liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The resulting LC-MS/MS data are used to identify peptides and thereby infer proteins present in samples ranging from simple mixtures to very complex proteomes. Bottom-up proteome studies usually involve quantitative comparisons a ...[more]