Ontology highlight
ABSTRACT:
SUBMITTER: Akhtar J
PROVIDER: S-EPMC6653780 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Akhtar Junaid J More Piyush P Albrecht Steffen S Marini Federico F Kaiser Waldemar W Kulkarni Apurva A Wojnowski Leszek L Fontaine Jean-Fred JF Andrade-Navarro Miguel A MA Silies Marion M Berger Christian C
Life science alliance 20190722 4
Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approac ...[more]