Dataset Information

De novo assembly and discovery of genes involved in the response of Solanum sisymbriifolium to Verticillium dahlia.

ABSTRACT: Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a devastating disease of eggplant (Solanum spp.) and causes substantial losses worldwide. Although some genes or biological processes involved in the interaction between eggplant and V. dahliae have been identified in some studies, the underlying molecular mechanism is not yet clear. Here, we monitored the transcriptomic profiles of the roots of resistant S. sisymbriifolium plants challenged with V. dahliae. Based on the measurements of physiological indexes (T-SOD, POD and SSs), three time points were selected and subsequently divided into two stages (S_12 h vs. S_0 h and S_48 h vs. S_12 h). KEGG enrichment analysis of the DEGs revealed several genes putatively involved in regulating plant-V. dahliae interactions, including mitogen-activated protein kinase (MAPK) genes (MEKK1 and MAP2K1), WRKY genes (WRKY22 and WRKY33) and cytochrome P450 (CYP) genes (CYP73A/C4H, CYP98A/C3'H and CYP84A/F5H). In addition, a subset of genes that play an important role in activating V. dahliae defence responses, including Ve genes as well as genes encoding PR proteins and TFs, were screened and are discussed. These results will help to identify key resistance genes and will contribute to a further understanding of molecular mechanisms of the S. sisymbriifolium resistance response to V. dahliae.

SUBMITTER: Wu L

PROVIDER: S-EPMC6656901 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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De novo assembly and discovery of genes involved in the response of Solanum sisymbriifolium to Verticillium dahlia.

Wu Liyan L Du Guanghui G Bao Rui R Li Zhibin Z Gong Yaju Y Liu Feihu F

Physiology and molecular biology of plants : an international journal of functional plant biology 20190513 4

Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a devastating disease of eggplant (Solanum spp.) and causes substantial losses worldwide. Although some genes or biological processes involved in the interaction between eggplant and V. dahliae have been identified in some studies, the underlying molecular mechanism is not yet clear. Here, we monitored the transcriptomic profiles of the roots of resistant S. sisymbriifolium plants challenged w ...[more]

PMID: 31402823

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Project description:BackgroundCamellia oleifera (C.oleifera) is one of the most important wood oil species in the world. C.oleifera was propagated by nurse seedling grafting. Since the morphology of rootstocks has a significant impact on grafting efficiency and seedling quality, it is necessary to understand the molecular mechanism of morphogenesis for cultivating high-quality and controllable rootstocks. However, the genomic resource for this species is relatively limited, which hinders us from fully understanding the molecular mechanisms of seed germination in C.oleifera.ResultsIn this paper, using transcriptome sequencing, we measured the gene expression in the C.oleifera cotyledon in different stages of development and the global gene expression profiles. Approximately 45.4 gigabases (GB) of paired-end clean reads were assembled into 113,582 unigenes with an average length of 396 bp. Six public protein databases annotate 61.5% (68,217) of unigenes. We identified 11,391 differentially expressed genes (DEGs) throughout different stages of germination. Enrichment analysis revealed that DEGs were mainly involved in hormone signal transduction and starch sucrose metabolism pathways. The gravitropism regulator UNE10, the meristem regulators STM, KNAT1, PLT2, and root-specific transcription factor WOX11 all have higher gene expression levels in the CAM2 stage (seed soaking), which indicates that the cotyledon-regulated program for germination had initiated when the seeds were imbibition. Our data showed differentially reprogrammed to multiple hormone-related genes in cotyledons during C.oleifera seed germination.ConclusionCotyledons play vital roles, both as the main nutrient provider and as one primary instructor for seed germination and seedling growth. Together, our study will significantly enrich the genomic resources of Camellia and help us understand the molecular mechanisms of the development in the seed germination and seedling growth of C.oleifera. It is helpful to culture standard and superior quality rootstock for C.oleifera breeding.

Dataset Information

De novo assembly and discovery of genes involved in the response of Solanum sisymbriifolium to Verticillium dahlia.

Publications

De novo assembly and discovery of genes involved in the response of <i>Solanum sisymbriifolium</i> to <i>Verticillium dahlia</i>.

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