Covalently-assembled single-chain protein nanostructures with ultra-high stability.

ABSTRACT: Protein nanostructures with precisely defined geometries have many potential applications in catalysis, sensing, signal processing, and drug delivery. While many de novo protein nanostructures have been assembled via non-covalent intramolecular and intermolecular interactions, a largely unexplored strategy is to construct nanostructures by covalently linking multiple individually folded proteins through site-specific ligations. Here, we report the synthesis of single-chain protein nanostructures with triangular and square shapes made using multiple copies of a three-helix bundle protein and split intein chemistry. Coarse-grained simulations confirm the experimentally observed flexibility of these nanostructures, which is optimized to produce triangular structures with high regularity. Thes

SUBMITTER: Bai W 

PROVIDER: S-EPMC6658521 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature